Luminescent lanthanide-binding peptides: sensitising the excited states of Eu(iii ) and Tb(iii ) with a 1,8-naphthalimide-based antenna
| العنوان: | Luminescent lanthanide-binding peptides: sensitising the excited states of Eu( |
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| المؤلفون: | Célia S. Bonnet, Thorfinnur Gunnlaugsson, Marc Devocelle |
| المساهمون: | Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Physiology and Medical Physics, School of Biochemistry and Immunology, Trinity College Dublin |
| المصدر: | Organic and Biomolecular Chemistry Organic and Biomolecular Chemistry, Royal Society of Chemistry, 2012, 10 (1), pp.126-33. ⟨10.1039/c1ob06567j⟩ |
| بيانات النشر: | Royal Society of Chemistry (RSC), 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Lanthanide, Magnetic Resonance Spectroscopy, MESH: Europium, MULTIWAVELENGTH SPECTROSCOPIC DATA, chemistry.chemical_element, Terbium, Peptide, 010402 general chemistry, Photochemistry, Lanthanoid Series Elements, 01 natural sciences, Biochemistry, AQUEOUS-SOLUTION, HIGH-AFFINITY, Europium, CHARGE-TRANSFER, MESH: Lanthanoid Series Elements, MESH: Terbium, AMINO-ACIDS, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Physical and Theoretical Chemistry, chemistry.chemical_classification, METAL-ION BINDING, MESH: Magnetic Resonance Spectroscopy, MESH: Peptides, 010405 organic chemistry, Chemistry, fungi, Organic Chemistry, TRYPTOPHAN-CONTAINING PARVALBUMIN, Nuclear magnetic resonance spectroscopy, Chromophore, Amides, MESH: Amides, 0104 chemical sciences, MESH: Luminescent Measurements, Excited state, Luminescent Measurements, CALCIUM-BINDING, EQUILIBRIUM-CONSTANTS, Peptides, ENERGY-TRANSFER, Luminescence |
| الوصف: | International audience; The investigation into the luminescence properties of a lanthanide-binding peptide, derived from the Ca-binding loop of the parvalbumin, and modified by incorporating a 1,8-naphthalimide (Naph) chromophore at the N-terminus is described. Here, the Naph is used as a sensitising antenna, which can be excited at lower energy than classical aromatic amino acids, such as tryptophan (the dodecapeptide of which was also synthesised and studied herein). The syntheses of the Naph antenna, its solid phase incorporation into the dodecapeptide, and the NMR investigation into the formation of the corresponding lanthanide complexes in solution is presented. We also show that this Naph antenna can be successfully employed to sensitize the excited states of both europium and terbium ions, the results of which was used to determined the stability constants of their formation complexes, and we demonstrated that our peptide 'loop' can selectively bind these lanthanide ions over Ca(II). |
| تدمد: | 1477-0539 1477-0520 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46d04a32780a0f98ca8dcbe97c2cf300 https://doi.org/10.1039/c1ob06567j |
| رقم الأكسشن: | edsair.doi.dedup.....46d04a32780a0f98ca8dcbe97c2cf300 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14770539 14770520 |
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