Protease Imaging of Human Atheromata Captures Molecular Information of Atherosclerosis, Complementing Anatomic Imaging
| العنوان: | Protease Imaging of Human Atheromata Captures Molecular Information of Atherosclerosis, Complementing Anatomic Imaging |
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| المؤلفون: | Dong-Eog Kim, Kwangmeyung Kim, Soo Min Shon, Ick Chan Kwon, Dong Kun Lee, Hyang Kyoung Kim, So Hyang Im, Dawid Schellingerhout, Jeongyeon Kim, Myoung Mook Lee, Sang Wuk Jeong, Eo Jin Kim, Geun Eun Kim, Seulki Lee |
| المصدر: | Arteriosclerosis, Thrombosis, and Vascular Biology. 30:449-456 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Pathology, medicine.medical_specialty, Computational biology, Cathepsin B, medicine, Humans, Carotid Stenosis, Prospective Studies, Aged, Fluorescent Dyes, Retrospective Studies, Ultrasonography, Endarterectomy, Carotid, business.industry, Matrix metalloproteinase 9, Molecular Imaging, Carotid Arteries, Matrix Metalloproteinase 9, Peptide Hydrolases, Matrix Metalloproteinase 2, Female, Stents, Molecular imaging, Cardiology and Cardiovascular Medicine, business, Structural imaging |
| الوصف: | Objective— There is hope that molecular imaging can identify vulnerable atherosclerotic plaques. However, there is a paucity of clinical translational data to guide the future development of this field. Here, we cross-correlate cathepsin-B or matrix metalloproteinase-2/-9 molecular optical imaging data of human atheromata or emboli with conventional imaging data, clinical data, and histopathologic data. Methods and Results— Fifty-two patients undergoing carotid endarterectomy (41 atheromata) or carotid stenting (15 captured emboli) were studied with protease-activatable imaging probes. We show that protease-related fluorescent signal in carotid atheromata or in emboli closely reflects the pathophysiologic alterations of plaque inflammation and statin-mediated therapeutic effects on plaque inflammation. Inflammation-related fluorescent signal was observed in the carotid bifurcation area and around ulcero-hemorrhagic lesions. Pathologically proven unstable plaques had high cathepsin-B–related fluorescent signal. The distribution patterns of the mean cathepsin-B imaging signals showed a difference between the symptomatic vs asymptomatic plaque groups. However, the degree of carotid stenosis or ultrasonographic echodensity was weakly correlated with the inflammatory proteolytic enzyme-related signal, suggesting that molecular imaging yields complimentary new information not available to conventional imaging. Conclusion— These results could justify and facilitate clinical trials to evaluate the use of protease-sensing molecular optical imaging in human atherosclerosis patients. |
| تدمد: | 1524-4636 1079-5642 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::477967eda62ddb33b7b4387b5910a3a6 https://doi.org/10.1161/atvbaha.109.194613 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....477967eda62ddb33b7b4387b5910a3a6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244636 10795642 |
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