Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar
| العنوان: | Mineral Trioxide Aggregate (MTA) Upregulates the Expression of DMP1 in Direct Pulp Capping in the Rat Molar |
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| المؤلفون: | Yoshihiro Shibukawa, Motohiko Nagayama, Satoshi Kawano, Michiko Ehara, Masashi Tanaka, Juna Nakao, Maiko Yamada, Takanaga Ochiai, Yuka Miyamoto, Yoshiaki Takitani, Takakazu Yoshida |
| المصدر: | Materials, Vol 14, Iss 4640, p 4640 (2021) Materials Volume 14 Issue 16 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Mineral trioxide aggregate, Technology, Dentin Matrix Acidic Phosphoprotein 1, Odontoblast differentiation, dentin bridge, Article, stomatognathic system, Dental pulp stem cells, nestin, General Materials Science, dentin matrix acidic phosphoprotein 1 (DMP1), mineral trioxide aggregate, Microscopy, QC120-168.85, Chemistry, QH201-278.5, Engineering (General). Civil engineering (General), Molecular biology, DMP1, Pulp capping, TK1-9971, Odontoblast, Descriptive and experimental mechanics, direct pulp capping, Pulp (tooth), mineral density, Electrical engineering. Electronics. Nuclear engineering, TA1-2040 |
| الوصف: | Mineral trioxide aggregate (MTA) is an alternative endodontic material that predicts conductive or inductive calcified tissue formation from immature pulp mesenchymal stem cells (IPMSCs). The purpose of this study was to investigate whether MTA could promote reparative odontoblast differentiation via IPMSCs in the early phase of regeneration and compare with calcium hydroxide (CH). Direct pulp capping using calcium hydroxide (CH), MTA, and MTA with platelet-rich plasma (MTA + PRP) was performed on maxillary first molars of 8-week-old male Wistar rats (n = 36). After 3, 7, or 14 days, the teeth were analyzed for mineral density (MD) and volume of MD (VMD) via micro-focusing computed tomography (µCT), nestin, dentin matrix acidic phosphoprotein 1 (DMP1) immunohistochemistry, and real-time PCR for DMP1 mRNA expression. MTA stimulated the early phase differentiation of the IPMSCs into odontoblasts, with positive results for nestin and DMP1 compared with CH. Moreover, MTA + PRP stimulated calcified granule and dentin bridge formation through calcium mineral deposition, following the induction of DMP1 mRNA expression in IPMSCs. Our results suggested that the combination of MTA and PRP is an effective and clinically applicable method for activating endogenous dental pulp stem cells into odontoblasts in the early stages of pulp regeneration. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1996-1944 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b103fb5c6906c2bd138075b3297407 https://www.mdpi.com/1996-1944/14/16/4640 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....47b103fb5c6906c2bd138075b3297407 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19961944 |
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