Anticancer Activity of Novel Plant Extracts and Compounds from Adenosma bracteosum (Bonati) in Human Lung and Liver Cancer Cells
| العنوان: | Anticancer Activity of Novel Plant Extracts and Compounds from Adenosma bracteosum (Bonati) in Human Lung and Liver Cancer Cells |
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| المؤلفون: | Van Giau Vo, Ngoc Hong Nguyen, Thi Ngoc Han Luong, Quang Thang Pham, Van Trung Phung, Qui Thanh Hoai Ta, Thuc-Huy Duong |
| المصدر: | Molecules Volume 25 Issue 12 Molecules, Vol 25, Iss 2912, p 2912 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | caspase-3, Ethyl acetate, Pharmaceutical Science, Pharmacology, Analytical Chemistry, lcsh:QD241-441, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, lcsh:Organic chemistry, Ursolic acid, Drug Discovery, medicine, Adenosma bracteosum, Physical and Theoretical Chemistry, Cytotoxicity, extract, anti-cancer, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Reactive oxygen species, Chloroform, Ethanol, Organic Chemistry, cell line, medicine.disease, isolated compounds, chemistry, Chemistry (miscellaneous), Cell culture, 030220 oncology & carcinogenesis, Large-cell lung carcinoma, Molecular Medicine |
| الوصف: | Cancer is the second leading cause of death globally, and despite the advances in drug development, it is still necessary to develop new plant-derived medicines. Compared with using conventional chemical drugs to decrease the side effects induced by chemotherapy, natural herbal medicines have many advantages. The present study aimed to discover the potential cytotoxicity of ethanol extract and its derived fractions (chloroform, ethyl acetate, butanol, and aqueous) of Adenosma bracteosum Bonati. (A. bracteosum) on human large cell lung carcinoma (NCI-H460) and hepatocellular carcinoma (HepG2). Among these fractions, the chloroform showed significant activity in the inhibition of proliferation of both cancerous cells because of the presence of bioactive compounds including xanthomicrol, 5,4&rsquo dihydroxy-6,7,8,3&rsquo tetramethoxyflavone, and ursolic acid which were clearly revealed by nuclear magnetic resonance spectroscopy (1H-NMR, 13C-NMR, Heteronuclear Multiple Bond Coherence, and Heteronuclear Single Quantum Coherence Spectroscopy) analyses. According to the radical scavenging capacity, the 5,4&rsquo tetramethoxyflavone compound (AB2) exhibited the highest anticancer activity on both NCI-H460 and HepG2 with IC50 values of 4.57 ± 0.32 and 5.67 ± 0.09 µ g/mL respectively, followed by the ursolic acid with the lower percent inhibition at 13.05 ± 0.55 and 10.00 ± 0.16 µ g/mL, respectively (p < 0.05). Remarkably, the AB2 compound induced to significant increase in the production of reactive oxygen species accompanied by attenuation of mitochondrial membrane potential, thus inducing the activation of caspase-3 activity in both human lung and liver cancer cells. These results suggest that A. bracteosum is a promising source of useful natural products and AB2 offers opportunities to develop the novel anticancer drugs. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1420-3049 |
| DOI: | 10.3390/molecules25122912 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::483d6fe9d8fa09144e11bc89290ccf3d |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....483d6fe9d8fa09144e11bc89290ccf3d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14203049 |
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| DOI: | 10.3390/molecules25122912 |