Effects of Wnt/β-catenin signaling on bisphenol A exposure in male mouse reproductive cells
| العنوان: | Effects of Wnt/β-catenin signaling on bisphenol A exposure in male mouse reproductive cells |
|---|---|
| المؤلفون: | Xianbin Song, Xiaosi Yang, Xiaoyu Chen, Zhiqi Fang, Xiaoli Liu |
| المصدر: | Molecular Medicine Reports. 12:5561-5567 |
| بيانات النشر: | Spandidos Publications, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, endocrine system, Cancer Research, medicine.medical_specialty, Litter Size, Air Pollutants, Occupational, Biology, Biochemistry, Mice, Phenols, Western blot, Pregnancy, Internal medicine, Genetics, medicine, Animals, Endocrine system, Sex Ratio, Benzhydryl Compounds, Spermatogenesis, Wnt Signaling Pathway, Molecular Biology, beta Catenin, Mice, Inbred ICR, Oncogene, medicine.diagnostic_test, urogenital system, Reproduction, Wnt signaling pathway, Gene Expression Regulation, Developmental, Leydig Cells, Environmental Exposure, Seminiferous Tubules, Cell cycle, Molecular medicine, Endocrinology, Oncology, Apoptosis, Prenatal Exposure Delayed Effects, Intercellular Signaling Peptides and Proteins, Molecular Medicine, Immunohistochemistry, Female |
| الوصف: | Bisphenol A (BPA) is a chemical used in numerous consumer products that is able to interfere with the mammalian endocrine system. The aim of the present study was to investigate the effects of BPA on male mouse reproductive cells following prenatal to postnatal exposure. In addition, the influence of BPA was detected on the expression levels of β‑catenin and dickkopf WNT signaling pathway inhibitor 1 (DKK‑1) during the differentiation of spermatogenic cells in the mouse testes. β‑catenin and DKK‑1 are two important proteins of the Wnt/β‑catenin signaling pathway. On gestational day 1, pregnant ICR mice were randomly divided into four groups: A dimethyl sulfoxide group, and three groups treated with various concentrations of BPA (0.5, 10, and 50 µg/kg). BPA was administered from gestational day 1 to weaning on postnatal day (PND) 42. The number of murine pups and the male:female ratio was recorded for each group. On PND 42, the male pups were sacrificed and their wet weights and testicular coefficients were measured. Immunohistochemical and western blot analyses were used to detect the protein expression of β‑catenin and DKK‑1 in the testicular tissue samples of the six‑week‑old male mice. The results indicated that the number of murine pups, as well as the testicular viscera coefficient of the male mice, significantly decreased in the BPA‑treated groups, as compared with the control group (P0.05, P0.01); however, no significant difference was observed in the male/female ratio in the BPA‑treated groups, as compared with the control group (P0.05). The results from the immunohistochemical and western blot analyses indicated that the protein expression of β‑catenin and DKK‑1 were significantly increased in the BPA‑treated groups, as compared with the control group, and the distribution of spermospore and Leydig cells also increased in the testes. These results suggest that high expression levels of β‑catenin and DKK‑1 may participate in BPA‑induced pathogenesis in male mouse reproductive cells. |
| تدمد: | 1791-3004 1791-2997 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::48b6a5507bda8b706dd8bac74a1ea97f https://doi.org/10.3892/mmr.2015.4028 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....48b6a5507bda8b706dd8bac74a1ea97f |
| قاعدة البيانات: | OpenAIRE |
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