Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme inXenopusgastrulae
| العنوان: | Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme inXenopusgastrulae |
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| المؤلفون: | Patrick Lemaire, Hitoyoshi Yasuo |
| المصدر: | Development. 128:3783-3793 |
| بيانات النشر: | The Company of Biologists, 2001. |
| سنة النشر: | 2001 |
| مصطلحات موضوعية: | Blastomeres, Embryo, Nonmammalian, Xenopus, Xenopus Proteins, Notochord formation, Mesoderm, Xenopus laevis, 0302 clinical medicine, beta Catenin, Genetics, 0303 health sciences, Wnt signaling pathway, Gene Expression Regulation, Developmental, Forkhead Transcription Factors, Cell biology, medicine.anatomical_structure, Bone Morphogenetic Proteins, embryonic structures, Intercellular Signaling Peptides and Proteins, Female, Signal Transduction, animal structures, LIM-Homeodomain Proteins, Molecular Sequence Data, Notochord, Biology, Bone morphogenetic protein, 03 medical and health sciences, Proto-Oncogene Proteins, medicine, Animals, Amino Acid Sequence, Molecular Biology, Glycoproteins, 030304 developmental biology, Homeodomain Proteins, Proteins, Gastrula, Zebrafish Proteins, biology.organism_classification, Frizzled Receptors, Repressor Proteins, Wnt Proteins, Gastrulation, Cytoskeletal Proteins, Goosecoid Protein, Somite, Frzb, Trans-Activators, 030217 neurology & neurosurgery, Transcription Factors, Developmental Biology |
| الوصف: | The Xenopus trunk organiser recruits neighbouring tissues into secondary trunk axial and paraxial structures and itself differentiates into notochord. The inductive properties of the trunk organiser are thought to be mediated by the secretion of bone morphogenetic protein (BMP) antagonists. Ectopic repression of BMP signals on the ventral side is sufficient to mimic the inductive properties of the trunk organiser. Resultant secondary trunks contain somite and neural tube, but no notochord.We show that inhibition of BMP signalling is sufficient for the initiation of the trunk organiser genetic programme at the onset of gastrulation. During late gastrulation, however, this programme is lost, due to an invasion of secreted Wnts from neighbouring tissues. Maintenance of this programme requires co-repression of BMP and Wnt signalling within the presumptive notochord region. To shed light on the molecular cascade that leads to the repression of the Wnt pathway, we looked for individual organiser genes whose overexpression could complement the inhibition of BMP signalling to promote notochord formation in the secondary trunks. Two genes, gsc and Xnot, were thus identified and shown to act in different ways. Xnot acts as a transcriptional repressor within the mesodermal region. Gsc acts in deeper vegetal cells, where it regulates Frzb expression to maintain Xnot expression in the neighbouring notochord territory.These results suggest that, during gastrulation, the necessary repression of Wnt/β-catenin signalling in notochord precursors is achieved by the action of secreted inhibitors, such as Frzb, emitted by gsc-expressing dorsal vegetal cells. |
| تدمد: | 1477-9129 0950-1991 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a6cc4d13a08a560887c27eba6128447 https://doi.org/10.1242/dev.128.19.3783 |
| رقم الأكسشن: | edsair.doi.dedup.....4a6cc4d13a08a560887c27eba6128447 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14779129 09501991 |
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