Revealing the Critical Role of Probe Grafting Density in Nanometric Confinement in Ionic Signal via an Experimental and Theoretical Study
| العنوان: | Revealing the Critical Role of Probe Grafting Density in Nanometric Confinement in Ionic Signal via an Experimental and Theoretical Study |
|---|---|
| المؤلفون: | Ranhao Xu, Qiujiao Du, Tianle Liu, Qun Ma, Fan Xia, Pengcheng Gao |
| المصدر: | Analytical Chemistry. 93:1984-1990 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Work (thermodynamics), Surface Properties, Chemistry, 010401 analytical chemistry, Charge density, Ionic bonding, DNA, Electrolyte, Models, Theoretical, 010402 general chemistry, Grafting, 01 natural sciences, Signal, Nanostructures, 0104 chemical sciences, Analytical Chemistry, Characterization (materials science), Chemical physics, Particle Size, DNA Probes, Electrodes, Nanoscopic scale, Oligonucleotide Array Sequence Analysis |
| الوصف: | The grafting density of probes at sensor interface plays a critical role in the performance of biochemical sensors. However, compared with macroscopic interface, the effects of probe grafting density at nanometric confinement are rarely studied due to the limitation of precise grafting density regulation and characterization at the nanoscale. Here, we investigate the effect from the grafting density of DNA probes on ionic signal for nucleic acid detection in a cylindrical nanochannel array (with diameter of 25 nm) by combing experiments and theories. We set up a theoretical model of charge distribution from close to inner wall of nanochannels at low probe grafting density to spreading in whole space at high probe grafting density. The theoretical results fit well with the experimental results. A reverse of ionic output from signal-off to signal-on occurs with increasing probe grafting density. Low probe grafting density offers a high current change ratio that is further enhanced using long-chain DNA probes or the electrolyte with a low salt concentration. This work develops an approach to enhance performance of nanochannel-based sensors and explore physicochemical properties in nanometric confines. |
| تدمد: | 1520-6882 0003-2700 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cabe3b748c72853ed2376abafa1b010 https://doi.org/10.1021/acs.analchem.0c03090 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4cabe3b748c72853ed2376abafa1b010 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15206882 00032700 |
|---|