Generation of a three-dimensional collagen scaffold-based model of the human endometrium
| العنوان: | Generation of a three-dimensional collagen scaffold-based model of the human endometrium |
|---|---|
| المؤلفون: | Margherita Y. Turco, Ridma C. Fernando, Yassen Abbas, Ruth E. Cameron, Michael Hollinshead, Lucia G Brunel, Lucy Gardner, Graham J. Burton, Ashley Moffett, Imogen Duncan, Serena M. Best |
| المساهمون: | Abbas, Yassen [0000-0001-8052-6691], Brunel, Lucia G [0000-0003-0327-5635], Apollo - University of Cambridge Repository |
| المصدر: | Interface Focus |
| بيانات النشر: | The Royal Society, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Cell type, Scaffold, Stromal cell, education, Biomedical Engineering, Biophysics, Bioengineering, Endometrium, Biochemistry, Biomaterials, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, medicine, Organoid, health care economics and organizations, 030304 developmental biology, 0303 health sciences, 030219 obstetrics & reproductive medicine, Chemistry, Cilium, Decidualization, Articles, Cell biology, Organoids, medicine.anatomical_structure, Collagen Scaffolds, Co-culture, Research Article, Biotechnology |
| الوصف: | The endometrium is the secretory lining of the uterus that undergoes dynamic changes throughout the menstrual cycle in preparation for implantation and a pregnancy. Recently, endometrial organoids (EO) were established to study the glandular epithelium. We have built upon this advance and developed a multi-cellular model containing both endometrial stromal and epithelial cells. We use porous collagen scaffolds produced with controlled lyophilization to direct cellular organization, integrating organoids with primary isolates of stromal cells. The internal pore structure of the scaffold was optimized for stromal cell culture in a systematic study, finding an optimal average pore size of 101 µm. EO seeded organize to form a luminal-like epithelial layer, on the surface of the scaffold. The cells polarize with their apical surface carrying microvilli and cilia that face the pore cavities and their basal surface attaching to the scaffold with the formation of extracellular matrix proteins. Both cell types are hormone responsive on the scaffold, with hormone stimulation resulting in epithelial differentiation and stromal decidualization. |
| وصف الملف: | application/pdf; application/vnd.openxmlformats-officedocument.wordprocessingml.document |
| تدمد: | 2042-8901 2042-8898 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4df9ff6cb8739e0f85a7ad89158fa204 https://doi.org/10.1098/rsfs.2019.0079 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....4df9ff6cb8739e0f85a7ad89158fa204 |
| قاعدة البيانات: | OpenAIRE |
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