An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies
| العنوان: | An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies |
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| المؤلفون: | Catherine Picart, Jacques Ohayon, Youri Arntz, Philippe Tracqui, Gérard Finet, Thomas Boudou |
| المساهمون: | Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Dynacell, VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Department of Hemodynamics and Interventional Cardiology, Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire des matériaux et du génie physique (LMGP ), Institut National Polytechnique de Grenoble (INPG)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), DynaCell |
| المصدر: | Journal of Biomechanics Journal of Biomechanics, Elsevier, 2006, 39 (9), pp.1677-85. ⟨10.1016/j.jbiomech.2005.04.026⟩ |
| بيانات النشر: | HAL CCSD, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Materials science, MESH: Gels, [SDV]Life Sciences [q-bio], 0206 medical engineering, Acrylic Resins, Biomedical Engineering, Biophysics, Aggregate modulus, Young's modulus, 02 engineering and technology, MESH: Imaging, Three-Dimensional, Suction, Models, Biological, 03 medical and health sciences, symbols.namesake, Imaging, Three-Dimensional, Dynamic modulus, Orthopedics and Sports Medicine, Elastic modulus, 030304 developmental biology, MESH: Acrylic Resins, 0303 health sciences, Bulk modulus, Rehabilitation, Linear elasticity, Mathematical analysis, MESH: Models, Biological, Adhesiveness, MESH: Suction, 020601 biomedical engineering, Elasticity, Poisson's ratio, Tangent modulus, symbols, MESH: Elasticity, MESH: Adhesiveness, Gels |
| الوصف: | International audience; The micropipette aspiration (MA) experiment remains a quite widely used micromanipulation technique for quantifying the elastic modulus of cells and, less frequently, of other biological samples. However, moduli estimations derived from MA experiments are only valid if the probed sample is non-adherent to the rigid substrate. This study extends this standard formulation by taking into account the influence of the sample adhesion. Using a finite element analysis of the sample aspiration into the micropipette, we derived a new expression of the aspirated length for linear elastic materials. Our results establish that (i) below a critical value, the thickness h of the probed sample must be considered to get an accurate value of its Young's modulus (ii) this critical value depends both on the Poisson's ratio and on the sample adhesivity. Additionally, we propose a novel method which allows the computation of the intrinsic Young's modulus of the adherent probed sample from its measured apparent elasticity modulus. Thanks to the set of computational graphs we derived from our theoretical analysis, we successfully validate this method by experiments performed on polyacrylamide gels. Interestingly, the original procedure we proposed allows a simultaneous quantification of the Young's modulus and of the Poisson's ratio of the adherent gel. Thus, our revisited analysis of MA experiments extends the application domain of this technique, while contributing to decrease the dispersion of elastic modulus values obtained by this method. |
| اللغة: | English |
| تدمد: | 0021-9290 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fe857065b20c10a1dbf88dc0734c515 https://hal.archives-ouvertes.fr/hal-00846411 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....4fe857065b20c10a1dbf88dc0734c515 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219290 |
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