Effective apical infection of differentiated human bronchial epithelial cells and induction of proinflammatory chemokines by the highly pneumotropic human adenovirus type 14p1
| العنوان: | Effective apical infection of differentiated human bronchial epithelial cells and induction of proinflammatory chemokines by the highly pneumotropic human adenovirus type 14p1 |
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| المؤلفون: | Thomas Dobner, Gregor Warnecke, Verena Kopfnagel, Sonja Schrepfer, Albert Heim, Elena Lam, Mirja Ramke |
| المصدر: | Schrepfer, Sonja; Lam, E; Ramke, M; Warnecke, G; Kopfnagel, V; Dobner, T; et al.(2015). Effective apical infection of differentiated human bronchial epithelial cells and induction of proinflammatory chemokines by the highly pneumotropic human adenovirus type 14p1. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/8mz6v2st PLoS ONE, Vol 10, Iss 7, p e0131201 (2015) PLoS ONE |
| بيانات النشر: | eScholarship, University of California, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | ARDS, Chemokine, Fluorescent Antibody Technique, lcsh:Medicine, Bronchi, Cell Separation, Biology, Virus, Proinflammatory cytokine, Adenovirus Infections, Human, medicine, Humans, Respiratory system, lcsh:Science, Desmoglein 2, Multidisciplinary, Respiratory tract infections, Adenoviruses, Human, lcsh:R, Virion, virus diseases, Cell Differentiation, Epithelial Cells, medicine.disease, eye diseases, medicine.anatomical_structure, Cell culture, Immunology, biology.protein, lcsh:Q, Chemokines, Inflammation Mediators, Research Article, Respiratory tract |
| الوصف: | Background Only a few pneumotropic types of the human adenoviruses (e.g. type B14p1) cause severe lower respiratory tract infections like pneumonia and acute respiratory distress syndrome (ARDS) even in immunocompetent patients. By contrast, many other human adenovirus (HAdV) types (e.g. HAdV-C5) are associated mainly with upper respiratory tract infections. This is in accordance with a highly physiological cell culture system consisting of differentiated primary human bronchial epithelial cells which are little susceptible for apical HAdV-C5 infections. Objective and Methods We hypothesized that a pneumotropic and highly pathogenic HAdV type infects differentiated human bronchial epithelial cells efficiently from the apical surface and also induces proinflammatory cytokines in order to establish ARDS and pneumonia. Therefore, the apical infection of differentiated primary human bronchial epithelial cells with the pneumotropic and virulent type HAdV-B14p1 was investigated in comparison to the less pneumotropic HAdV-C5 as a control. Results Binding of HAdV-B14p1 to the apical surface of differentiated human bronchial epithelial cells and subsequent internalization of HAdV DNA was 10 fold higher (p |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::506f89b19da7adccb3c4062ec75d9710 http://www.escholarship.org/uc/item/8mz6v2st |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....506f89b19da7adccb3c4062ec75d9710 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |