A Comparison of Three Methods for the Detection of Circulating Tumor Cells in Patients with Early and Metastatic Breast Cancer
العنوان: | A Comparison of Three Methods for the Detection of Circulating Tumor Cells in Patients with Early and Metastatic Breast Cancer |
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المؤلفون: | Giannis Stoupis, Maria Spiliotaki, Dimitrios Mavroudis, Stella Apostolaki, M. Perraki, Vassilis Georgoulias, Filippos Koinis, Eleni Politaki, Galatea Kallergi, Evi Lianidou, Georgia Saloustrou, Dora Hatzidaki, Areti Strati, Tereza Skaltsi, Sofia Agelaki |
المصدر: | Cellular Physiology and Biochemistry, Vol 44, Iss 2, Pp 594-606 (2017) |
بيانات النشر: | S. Karger AG, 2017. |
سنة النشر: | 2017 |
مصطلحات موضوعية: | 0301 basic medicine, CA15-3, Oncology, Physiology, medicine.medical_treatment, Immunofluorescence, Kaplan-Meier Estimate, lcsh:Physiology, 0302 clinical medicine, Circulating tumor cell, lcsh:QD415-436, Neoplasm Metastasis, Real Time RT-qPCR, Aged, 80 and over, lcsh:QP1-981, Middle Aged, Neoplastic Cells, Circulating, Prognosis, Metastatic breast cancer, 030220 oncology & carcinogenesis, Female, Cell Search System, Adjuvant, Adult, medicine.medical_specialty, Concordance, CA 15-3, Breast Neoplasms, Real-Time Polymerase Chain Reaction, lcsh:Biochemistry, 03 medical and health sciences, Breast cancer, Cell Line, Tumor, Internal medicine, Breast Cancer, medicine, Humans, RNA, Messenger, Aged, Keratin-19, Chemotherapy, Keratin-18, business.industry, Keratin-8, medicine.disease, Early Diagnosis, 030104 developmental biology, Microscopy, Fluorescence, Leukocyte Common Antigens, business, Circulating Tumor Cells |
الوصف: | Background: We directly compared CTC detection rates and prognostic significance, using three different methods in patients with breast cancer (BC). Methods: Early (n=200) and metastatic (n=164) patients were evaluated before initiating adjuvant or first-line chemotherapy, using the CellSearchTM System, an RT-qPCR for CK-19 mRNA detection and by double immunofluorescence (IF) microscopy using A45-B/B3 and CD45 antibodies. Results: Using the CellSearchTM System, 37% and 16.5% of early BC patients were CTC-positive (at ≥1 and ≥2 CTCs/23 ml of blood), 18.0% by RT-qPCR and 16.9% by IF; no agreement was observed between methods. By the CellSearchTM 34.8% and 53.7% (at≥ 5 and ≥ 2 CTCs/7.5 ml) of metastatic patients were CTC-positive, 37.8% by RT-qPCR and 28.5% by IF. A significant agreement existed only between the CellSearchTM and RT-qPCR. In 60.8% of cases, differential EpCAM and CK-19 expression on CTCs by IF could explain the discrepancies between the CellSearchTM and RT-qPCR. CTC-positivity by either method was associated with decreased overall survival in metastatic patients. Conclusion: A significant concordance was observed between the CellSearchTM and RT-qPCR in metastatic but not in early BC. Discordant results could be explained in part by CTC heterogeneity. CTC detection by all methods evaluated had prognostic relevance in metastatic patients. |
تدمد: | 1421-9778 1015-8987 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50b142f629575decf680ea57172c3aff https://doi.org/10.1159/000485115 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....50b142f629575decf680ea57172c3aff |
قاعدة البيانات: | OpenAIRE |
تدمد: | 14219778 10158987 |
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