Neutrophil Extracellular Traps Entrap and Kill Borrelia burgdorferi Sensu Stricto Spirochetes and Are Not Affected by Ixodes ricinus Tick Saliva
| العنوان: | Neutrophil Extracellular Traps Entrap and Kill Borrelia burgdorferi Sensu Stricto Spirochetes and Are Not Affected by Ixodes ricinus Tick Saliva |
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| المؤلفون: | Catherine Menten-Dedoyart, Céline Faccinetto, Christophe Desmet, Sophie Dubois, Pierre-Bernard Van Lerberghe, Nataliia Rudenko, Maryna Golovchenko, Bernard Couvreur, Frédéric Baron, Cécile Oury, Benaïssa Elmoualij, Ernst Heinen, Ingrid Dupiereux |
| المصدر: | The Journal of Immunology. 189:5393-5401 |
| بيانات النشر: | The American Association of Immunologists, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Ixodes ricinus, Neutrophils, Immunology, Tick, Microbiology, Mice, Lyme disease, Borrelia burgdorferi Group, Borrelia, parasitic diseases, medicine, Animals, Humans, Immunology and Allergy, Bites and Stings, Borrelia burgdorferi, Saliva, Lyme Disease, Ixodes, biology, DNA, Neutrophil extracellular traps, bacterial infections and mycoses, biology.organism_classification, medicine.disease, Virology, Glossitis, Benign Migratory, Respiratory burst, Neutrophil Infiltration, Lyme disease microbiology, Arachnid Vectors, Female, Rabbits, Reactive Oxygen Species |
| الوصف: | Lyme disease is caused by spirochetes of the Borrelia burgdorferi sensu lato complex. They are transmitted mainly by Ixodes ricinus ticks. After a few hours of infestation, neutrophils massively infiltrate the bite site. They can kill Borrelia via phagocytosis, oxidative burst, and hydrolytic enzymes. However, factors in tick saliva promote propagation of the bacteria in the host even in the presence of a large number of neutrophils. The neutrophil extracellular trap (NET) consists in the extrusion of the neutrophil’s own DNA, forming traps that can retain and kill bacteria. The production of reactive oxygen species is apparently associated with the onset of NETs (NETosis). In this article, we describe NET formation at the tick bite site in vivo in mice. We show that Borrelia burgdorferi sensu stricto spirochetes become trapped and killed by NETs in humans and that the bacteria do not seem to release significant nucleases to evade this process. Saliva from I. ricinus did not affect NET formation by human neutrophils or its stability. However, it greatly decreased neutrophil reactive oxygen species production, suggesting that a strong decrease of hydrogen peroxide does not affect NET formation. Finally, round bodies trapped in NETs were observed, some of them staining as live bacteria. This observation could help contribute to a better understanding of the early steps of Borrelia invasion and erythema migrans formation after tick bite. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52343395b9b103e75031b8dd6078ee47 https://doi.org/10.4049/jimmunol.1103771 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....52343395b9b103e75031b8dd6078ee47 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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