Changes in nonstructural protein 3 are associated with attenuation in avian coronavirus infectious bronchitis virus
| العنوان: | Changes in nonstructural protein 3 are associated with attenuation in avian coronavirus infectious bronchitis virus |
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| المؤلفون: | N. D. Acevedol, Jon S. Robertson, Jamie E. Phillips, Sharmi Thor, Cornelia Lemke, Jessica C. Kissinger, Andrew H. Paterson, Susan M. Williams, Mark W. Jackwood, Enid T. McKinley, Deborah A. Hilt |
| المصدر: | Virus Genes |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Serotype, medicine.medical_specialty, animal structures, Infectious bronchitis virus, Molecular Sequence Data, Virulence, Chick Embryo, Viral Nonstructural Proteins, Biology, Virus Replication, Genome, Article, Virus, Microbiology, Medical microbiology, Virology, Genetics, medicine, Animals, Pathogenicity, Molecular Biology, Phylogeny, Poultry Diseases, Comparative genomics, Avian coronavirus, Embryonated, Attenuation, General Medicine, Viral replication, embryonic structures, Molecular evolution, Coronavirus Infections, Chickens |
| الوصف: | Full-length genome sequencing of pathogenic and attenuated (for chickens) avian coronavirus infectious bronchitis virus (IBV) strains of the same serotype was conducted to identify genetic differences between the pathotypes. Analysis of the consensus full-length genome for three different IBV serotypes (Ark, GA98, and Mass41) showed that passage in embryonated eggs, to attenuate the viruses for chickens, resulted in 34.75–43.66% of all the amino acid changes occurring in nsp 3 within a virus type, whereas changes in the spike glycoprotein, thought to be the most variable protein in IBV, ranged from 5.8 to 13.4% of all changes. The attenuated viruses did not cause any clinical signs of disease and had lower replication rates than the pathogenic viruses of the same serotype in chickens. However, both attenuated and pathogenic viruses of the same serotype replicated similarly in embryonated eggs, suggesting that mutations in nsp 3, which is involved in replication of the virus, might play an important role in the reduced replication observed in chickens leading to the attenuated phenotype. Electronic supplementary material The online version of this article (doi:10.1007/s11262-011-0668-7) contains supplementary material, which is available to authorized users. |
| تدمد: | 1572-994X 0920-8569 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52a06a789d30f9149e9ae3b7ec746725 https://doi.org/10.1007/s11262-011-0668-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....52a06a789d30f9149e9ae3b7ec746725 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1572994X 09208569 |
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