RNA-Binding Proteomics Reveals MATR3 Interacting with lncRNA SNHG1 To Enhance Neuroblastoma Progression
| العنوان: | RNA-Binding Proteomics Reveals MATR3 Interacting with lncRNA SNHG1 To Enhance Neuroblastoma Progression |
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| المؤلفون: | Chiao Hui Hsieh, Tz Wen Yang, Hsueh Fen Juan, Hsuan Cheng Huang, Chia-Lang Hsu, Divya Sahu, Yi Wen Chang |
| المصدر: | Journal of Proteome Research. |
| بيانات النشر: | American Chemical Society (ACS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Proteomics, 0301 basic medicine, RNA Splicing, Biology, Biochemistry, Neuroblastoma, 03 medical and health sciences, 0302 clinical medicine, Nuclear Matrix-Associated Proteins, Western blot, Cell Line, Tumor, medicine, Humans, Binding site, medicine.diagnostic_test, RNA-Binding Proteins, RNA, General Chemistry, medicine.disease, Survival Analysis, Cell biology, 030104 developmental biology, Rna immunoprecipitation, 030220 oncology & carcinogenesis, RNA splicing, Disease Progression, Ribonucleoprotein complex biogenesis, RNA, Long Noncoding, Protein Binding |
| الوصف: | The interaction of long noncoding RNAs (lncRNAs) with one or more RNA-binding proteins (RBPs) is important to a plethora of cellular and physiological processes. The lncRNA SNHG1 was reported to be aberrantly expressed and associated with poor patient prognosis in several cancers including neuroblastoma. However, the interacting RBPs and biological functions associated with SNHG1 in neuroblastoma remain unknown. In this study, we identified 283, 31, and 164 SNHG1-interacting proteins in SK-N-BE(2)C, SK-N-DZ, and SK-N-AS neuroblastoma cells, respectively, using a RNA-protein pull-down assay coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Twenty-four SNHG1-interacting RBPs were identified in common from these three neuroblastoma cell lines. RBPs MATR3, YBX1, and HNRNPL have the binding sites for SNHG1 predicted by DeepBind motif analysis. Furthermore, the direct binding of MATR3 with SNHG1 was validated by Western blot and confirmed by RNA immunoprecipitation assay (RIP). Coexpression analysis revealed that the expression of SNHG1 is positively correlated with MATR3 ( P = 3.402 × 10-13). The high expression of MATR3 is associated with poor event-free survival ( P = 0.00711) and overall survival ( P = 0.00064). Biological functions such as ribonucleoprotein complex biogenesis, RNA processing, and RNA splicing are significantly enriched and in common between SNHG1 and MATR3. In conclusion, we identified MATR3 as binding to SNHG1 and the interaction might be involved in splicing events that enhance neuroblastoma progression. |
| تدمد: | 1535-3907 1535-3893 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b0d3037562bac55934ce685cc978a14 https://doi.org/10.1021/acs.jproteome.8b00693 |
| رقم الأكسشن: | edsair.doi.dedup.....5b0d3037562bac55934ce685cc978a14 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15353907 15353893 |
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