Concurrent visualization of trafficking, expansion, and activation of T lymphocytes and T-cell precursors in vivo
| العنوان: | Concurrent visualization of trafficking, expansion, and activation of T lymphocytes and T-cell precursors in vivo |
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| المؤلفون: | Marcel R.M. van den Brink, Arnab Ghosh, Jennifer Tsai, Ronald G. Blasberg, John C. Markley, Alexander D. Klose, Nury L. Yim, Bradley J. Beattie, Elmer Santos, Inna Serganova, Il-Kang Na, Renier J. Brentjens, Amanda M. Holland, Matthias Stephan, Uttam K. Rao, Michel Sadelain |
| المصدر: | Blood. 116:e18-e25 |
| بيانات النشر: | American Society of Hematology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Male, Cell type, Adoptive cell transfer, Luminescence, T-Lymphocytes, Immunology, Graft vs Host Disease, Biology, Lymphocyte Activation, Biochemistry, Jurkat cells, Cell Line, Jurkat Cells, Mice, Cell Movement, Cell Line, Tumor, Animals, Humans, Luciferases, Promoter Regions, Genetic, Cell Proliferation, Mice, Inbred C3H, Precursor Cells, T-Lymphoid, NFATC Transcription Factors, Cell growth, Lentivirus, T-cell receptor, Hematopoietic Stem Cell Transplantation, NFAT, Gene Therapy, 3T3 Cells, Cell Biology, Hematology, Adoptive Transfer, Cell biology, Mice, Inbred C57BL, Luminescent Measurements, Female, CD8 |
| الوصف: | We have developed a dual bioluminescent reporter system allowing noninvasive, concomitant imaging of T-cell trafficking, expansion, and activation of nuclear factor of activated T cells (NFAT) in vivo. NFAT activation plays an important role in T-cell activation and T-cell development. Therefore we used this system to determine spatial-temporal activation patterns of (1) proliferating T lymphocytes during graft-versus-host disease (GVHD) and (2) T-cell precursors during T-cell development after allogeneic hematopoietic stem cell transplantation (HSCT). In the first days after HSCT, donor T cells migrated to the peripheral lymph nodes and the intestines, whereas the NFAT activation was dominant in the intestines, suggesting an important role for the intestines in the early stages of alloactivation during development of GVHD. After adoptive transfer of in vitro-derived T-cell receptor (TCR) H-Y transgenic T-cell precursors into B6 (H-2b) hosts of both sexes, NFAT signaling and development into CD4+ or CD8+ single-positive cells could only be detected in the thymus of female recipients indicating either absence of positive selection or prompt depletion of double-positive thymocytes in the male recipients. Because NFAT plays an important role in a wide range of cell types, our system could provide new insights into a variety of biologic processes. |
| تدمد: | 1528-0020 0006-4971 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b12032ad31b768a3c541484d0b2866a https://doi.org/10.1182/blood-2009-12-259432 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....5b12032ad31b768a3c541484d0b2866a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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