Identification of a phage-derived depolymerase specific for KL47 capsule of Klebsiella pneumoniae and its therapeutic potential in mice
العنوان: | Identification of a phage-derived depolymerase specific for KL47 capsule of Klebsiella pneumoniae and its therapeutic potential in mice |
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المؤلفون: | Min Li, Hui Wang, Long Chen, Genglin Guo, Pei Li, Jiale Ma, Rong Chen, Hong Du, Yuqing Liu, Wei Zhang |
المصدر: | Virologica Sinica. 37:538-546 |
بيانات النشر: | Elsevier BV, 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Klebsiella pneumoniae, Mice, Virology, Immunology, Animals, Molecular Medicine, Bacteriophages, Anti-Bacterial Agents, Klebsiella Infections |
الوصف: | Klebsiella pneumoniae is one of the major pathogens causing global multidrug-resistant infections. Therefore, strategies for preventing and controlling the infections are urgently needed. Phage depolymerase, often found in the tail fiber protein or the tail spike protein, is reported to have antibiofilm activity. In this study, phage P560 isolated from sewage showed specific for capsule locus type KL47 K. pneumoniae, and the enlarged haloes around plaques indicated that P560 encoded a depolymerase. The capsule depolymerase, ORF43, named P560dep, derived from phage P560 was expressed, purified, characterized and evaluated for enzymatic activity as well as specificity. We reported that the capsule depolymerase P560dep, can digest the capsule polysaccharides on the surface of KL47 type K. pneumoniae, and the depolymerization spectrum of P560dep matched to the host range of phage P560, KL47 K. pneumoniae. Crystal violet staining assay showed that P560dep was able to significantly inhibit biofilm formation. Further, a single dose (50 μg/mouse) of depolymerase intraperitoneal injection protected 90%-100% of mice from lethal challenge before or after infection by KL47 carbapenem-resistant K. pneumoniae. And pathological changes were alleviated in lung and liver of mice infected by KL47 type K. pneumoniae. It is demonstrated that depolymerase P560dep as an attractive antivirulence agent represents a promising tool for antimicrobial therapy. |
تدمد: | 1995-820X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dfd9e0bfbce8c15a3ba68385a45adb2 https://doi.org/10.1016/j.virs.2022.04.005 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....5dfd9e0bfbce8c15a3ba68385a45adb2 |
قاعدة البيانات: | OpenAIRE |
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