Goosecoid Controls Neuroectoderm Specification via Dual Circuits of Direct Repression and Indirect Stimulation in Xenopus Embryos
| العنوان: | Goosecoid Controls Neuroectoderm Specification via Dual Circuits of Direct Repression and Indirect Stimulation in Xenopus Embryos |
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| المؤلفون: | Ravi Shankar Goutam, Unjoo Lee, Zobia Umair, Jaebong Kim, Vijay Kumar, Shiv Kumar |
| المصدر: | Molecules and Cells |
| بيانات النشر: | Korean Society for Molecular and Cellular Biology, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Mesoderm, endocrine system, animal structures, Xenopus, Ectoderm, Gsc, Biology, Noggin, medicine, transcriptional regulation, Molecular Biology, NeuroD, Neuroectoderm, neuroectoderm, Neurogenesis, fungi, dorsal organizer, Cell Biology, General Medicine, Cell biology, medicine.anatomical_structure, Gsc response element, embryonic structures, Neural cell adhesion molecule, Chordin, chordin, Research Article |
| الوصف: | Spemann organizer is a center of dorsal mesoderm and itself retains the mesoderm character, but it has a stimulatory role for neighboring ectoderm cells in becoming neuroectoderm in gastrula embryos. Goosecoid (Gsc) overexpression in ventral region promotes secondary axis formation including neural tissues, but the role of gsc in neural specification could be indirect. We examined the neural inhibitory and stimulatory roles of gsc in the same cell and neighboring cells contexts. In the animal cap explant system, Gsc overexpression inhibited expression of neural specific genes including foxd4l1.1, zic3, ncam, and neurod. Genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) and promoter analysis of early neural genes of foxd4l1.1 and zic3 were performed to show that the neural inhibitory mode of gsc was direct. Site-directed mutagenesis and serially deleted construct studies of foxd4l1.1 promoter revealed that Gsc directly binds within the foxd4l1.1 promoter to repress its expression. Conjugation assay of animal cap explants was also performed to demonstrate an indirect neural stimulatory role for gsc. The genes for secretory molecules, Chordin and Noggin, were up-regulated in gsc injected cells with the neural fate only achieved in gsc uninjected neighboring cells. These experiments suggested that gsc regulates neuroectoderm formation negatively when expressed in the same cell and positively in neighboring cells via soluble factors. One is a direct suppressive circuit of neural genes in gsc expressing mesoderm cells and the other is an indirect stimulatory circuit for neurogenesis in neighboring ectoderm cells via secreted BMP antagonizers. |
| اللغة: | English |
| تدمد: | 0219-1032 1016-8478 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::648e61b4482de7720709a61abec1047a http://europepmc.org/articles/PMC8560583 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....648e61b4482de7720709a61abec1047a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02191032 10168478 |
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