Autophagy and mitochondrial dysfunction in adjuvant-arthritis rats treatment with resveratrol
العنوان: | Autophagy and mitochondrial dysfunction in adjuvant-arthritis rats treatment with resveratrol |
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المؤلفون: | Wei Cao, Jinseng Lu, Xianbin Song, Gaoyuan Wang, Junqiang Zhang, Xiaoyu Chen, Zhicheng Wang, Xiaoqing Wang |
المصدر: | Scientific Reports |
بيانات النشر: | Springer Science and Business Media LLC, 2016. |
سنة النشر: | 2016 |
مصطلحات موضوعية: | 0301 basic medicine, Glutathione reductase, Pharmacology, Resveratrol, Mitochondrion, medicine.disease_cause, Article, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Stilbenes, Autophagy, medicine, Animals, Cells, Cultured, chemistry.chemical_classification, Reactive oxygen species, Multidisciplinary, biology, Glutathione peroxidase, Anti-Inflammatory Agents, Non-Steroidal, Arthritis, Experimental, Synoviocytes, Molecular biology, Mitochondria, Rats, Oxidative Stress, 030104 developmental biology, chemistry, 030220 oncology & carcinogenesis, biology.protein, Reactive Oxygen Species, Oxidative stress |
الوصف: | Resveratrol is a polyphenol derivatives which exhibits a pro-apoptotic effect in a variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are scarcely reports on its apoptosis-promoting effect in abnormal proliferation fibroblast-like synoviocytes (FLSs). In this study, we investigated the underlying mechanism and apoptosis-inducing effects of resveratrol on the abnormal proliferation of FLSs in adjuvant-arthritis (AA) rats. Since using resveratrol for 12 days resulted in a significant decreasing the swelling degree of the paw, reducing malondialdehyde (MDA) content and enhancing superoxide dismutase (SOD) activity, antioxidant capacity, glutathione peroxidase and glutathione reductase ratio in AA rats. Moreover, we found that 5 μMH2O2 could increase cells viability, Beclin1, LC3A/B, MnSOD, SIRT3 protein expression in FLSs. But, resveratrol could reverse these effects by changing mitochondrial membrane potential (Δψm) to promote mitochondrial reactive oxygen species (mtROS) generation in 5 μMH2O2-treatment FLSs. These results suggest that oxidative stress existed in AA rats. Resveratrol could suppress oxidative stress in AA rats and increase mtROS production by reducing autophagy protein Beclin1, LC3A/B and oxidative stress protein MnSOD to promoted the apoptosis of FLSs. Thus, targeting of mtROS may be a crucial mechanism of resveratrol confers patients with rheumatoid arthritis. |
تدمد: | 2045-2322 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::65f3d3888c1ebdd2868c4ed190577aa2 https://doi.org/10.1038/srep32928 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....65f3d3888c1ebdd2868c4ed190577aa2 |
قاعدة البيانات: | OpenAIRE |
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