A deeper look at carrier proteome effects for single-cell proteomics
| العنوان: | A deeper look at carrier proteome effects for single-cell proteomics |
|---|---|
| المؤلفون: | Tanveer Singh Batth, Zilu Ye, Jesper Olsen, Patrick Leopold Rüther |
| المصدر: | Ye, Z, Batth, T S, Rüther, P & Olsen, J V 2022, ' A deeper look at carrier proteome effects for single-cell proteomics ', Communications Biology, vol. 5, 150 . https://doi.org/10.1038/s42003-022-03095-4 Commun Biol |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Proteomics, medicine.anatomical_structure, Proteome, Tandem Mass Spectrometry, Cell, medicine, Medicine (miscellaneous), Computational biology, Biology, General Agricultural and Biological Sciences, General Biochemistry, Genetics and Molecular Biology |
| الوصف: | Multiplexing approaches using tandem mass tags with a carrier proteome to boost sensitivity have advanced single cell proteomics by mass spectrometry (SCoPE-MS). Here, we probe the carrier proteome effects in single cell proteomics with mixed species TMTpro-labeled samples. We demonstrate that carrier proteomes, while increasing overall identifications, dictate which proteins are identified. We show that quantitative precision and signal intensity are limited at high carrier levels, hindering the recognition of regulated proteins. Guidelines for optimized mass spectrometry acquisition parameters and best practices for fold-change or protein copy number-based comparisons are provided. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::679a87af852e86b5e2b679f8d2518614 https://curis.ku.dk/ws/files/300772818/s42003_022_03095_4.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....679a87af852e86b5e2b679f8d2518614 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |