Heterogeneous EGFR, CDK4, MDM4, and PDGFRA gene expression profiles in primary GBM: No association with patient survival
| العنوان: | Heterogeneous EGFR, CDK4, MDM4, and PDGFRA gene expression profiles in primary GBM: No association with patient survival |
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| المؤلفون: | María Jara-Acevedo, María González-Tablas, Nerea González-García, Álvaro Otero, Ana B. Nieto-Librero, Daniel Pascual, Alberto Orfao, Carlos Prieto, María Dolores Tabernero, Laura Ruiz, Purificación Galindo-Villardón, Pablo Sousa, Hermínio Tão, Daniel Arandia, Ana-Luisa Vital |
| المساهمون: | Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), European Commission |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname Cancers Volume 12 Issue 1 Cancers, Vol 12, Iss 1, p 231 (2020) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Platelet-Derived Growth Factor Receptor Alpha, PDGFRA, amplification, lcsh:RC254-282, Article, 03 medical and health sciences, Exon, 0302 clinical medicine, Intragenic deletions, Gene expression, medicine, Epidermal growth factor receptor, Gene, Chromosome 7 (human), Polysomy, biology, glioblastoma, Gene expression profile, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, 030104 developmental biology, intragenic deletions, Oncology, 030220 oncology & carcinogenesis, Cancer research, biology.protein, gene expression profile, heterogeneity, Heterogeneity, Glioblastoma |
| الوصف: | This article belongs to the Special Issue Brain Tumors. [Background]: The prognostic impact of the expression profile of genes recurrently amplified in glioblastoma multiforme (GBM) remains controversial. [Methods]: We investigated the RNA gene expression profile of epidermal growth factor receptor (EGFR), cyclin-dependent kinase 4 (CDK4), murine doble minute 4 (MDM4), and platelet derived growth factor receptor alpha (PDGFRA) in 83 primary GBM tumors vs. 42 normal brain tissue samples. Interphase FISH (iFISH) analysis for the four genes, together with analysis of intragenic deletions in EGFR and PDGFRA, were evaluated in parallel at the DNA level. As validation cohort, publicly available RNA gene expression data on 293 samples from 10 different GBM patient series were also studied. [Results]: At the RNA level, CDK4 was the most frequently overexpressed gene (90%) followed by EGFR (58%) and PDGFRA (58%). Chromosome 7 copy number alterations, i.e., trisomy (49%) and polysomy (44%), showed no clear association with EGFR gene expression levels. In turn, intragenic EGFR deletions were found in 39 patients (47%), including EGFRvIII (46%) in association with EGFRvIVa (4%), EGFRvII (2%) or other EGFR deletions (3%) and PDGFRA deletion of exons 8–9 was found in only two tumors (2%). [Conclusions]: Overall, none of the gene expression profiles and/or intragenic EGFR deletions showed a significant impact on overall survival of GBM supporting the notion that other still unraveled features of the disease might play a more relevant prognostic role in GBM This research was funded by Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and Ministerio de Economía y Competitividad (RD12/0036/0048, AES PI16/00476-FONDOS FEDER and CB16/12/00400). |
| وصف الملف: | application/pdf |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6984f746c07b48d6cfc9bc541eea313f http://hdl.handle.net/10261/222861 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6984f746c07b48d6cfc9bc541eea313f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |