Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori
| العنوان: | Construction of Baculovirus-Inducible CRISPR/Cas9 Antiviral System Targeting BmNPV in Bombyx mori |
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| المؤلفون: | Yujia Liu, Dongbin Chen, Xiaoqian Zhang, Shuqing Chen, Dehong Yang, Linmeng Tang, Xu Yang, Yaohui Wang, Xingyu Luo, Manli Wang, Zhihong Hu, Yongping Huang |
| المصدر: | Viruses, Vol 14, Iss 59, p 59 (2022) Viruses; Volume 14; Issue 1; Pages: 59 Viruses |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | DNA Replication, sericulture, baculovirus-inducible CRISPR/Cas9, Gene Expression, Bombyx mori, BmNPV, antivirus, Virus Replication, Antiviral Agents, Microbiology, Article, Animals, Genetically Modified, Virology, Animals, Promoter Regions, Genetic, Gene Editing, fungi, Bombyx, Nucleopolyhedroviruses, QR1-502, Infectious Diseases, Virus Diseases, DNA, Viral, CRISPR-Cas Systems, Baculoviridae, RNA, Guide, Kinetoplastida |
| الوصف: | The silkworm Bombyx mori is an economically important insect. The sericulture industry is seriously affected by pathogen infections. Of these pathogens, Bombyx mori nucleopolyhedrovirus (BmNPV) causes approximately 80% of the total economic losses due to pathogen infections. We previously constructed a BmNPV-specific CRISPR/Cas9 silkworm line with significantly enhanced resistance to BmNPV. In order to optimize the resistance properties and minimize its impact on economic traits, we constructed an inducible CRISPR/Cas9 system for use in transgenic silkworms. We used the 39k promoter, which is induced by viral infection, to express Cas9 and the U6 promoter to express four small guide RNA targeting the genes encoding BmNPV late expression factors 1 and 3 (lef-1 and lef-3, respectively), which are essential for viral DNA replication. The system was rapidly activated when the silkworm was infected and showed considerably higher resistance to BmNPV infection than the wild-type silkworm. The inducible system significantly reduced the development effects due to the constitutive expression of Cas9. No obvious differences in developmental processes or economically important characteristics were observed between the resulting transgenic silkworms and wild-type silkworms. Adoption of this accurate and highly efficient inducible CRISPR/Cas9 system targeting BmNPV DNA replication will result in enhanced antivirus measures during sericulture, and our work also provides insights into the broader application of the CRISPR/Cas9 system in the control of infectious diseases and insect pests. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c9586283284295ccd7d31222ca50dba https://www.mdpi.com/1999-4915/14/1/59 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6c9586283284295ccd7d31222ca50dba |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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