Null Mutation ofAtCUL1Causes Arrest in Early Embryogenesis inArabidopsis
| العنوان: | Null Mutation ofAtCUL1Causes Arrest in Early Embryogenesis inArabidopsis |
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| المؤلفون: | Yves Parmentier, Aiwu Dong, Esther Lechner, Mark Estelle, Pascal Genschik, Loïc Lepiniec, Hanjo Hellmann, Wen-Hui Shen, Fabienne Granier, Jean Masson |
| المساهمون: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), University of Texas at Austin, Institut de Biologie des Plantes (IBP), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Fudan University (FU), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Laboratoire de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA) |
| المصدر: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2002, 13 (6), pp.1916-1928. ⟨10.1091/mbc.E02-02-0077⟩ |
| بيانات النشر: | American Society for Cell Biology (ASCB), 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | 0106 biological sciences, Cytoplasm, tobacco by2 cells, [SDV]Life Sciences [q-bio], Mutant, Arabidopsis, Cell Cycle Proteins, 01 natural sciences, higher-plants, cop9 signalosome, Morphogenesis, Cell division control protein 4, s-phase, degradation, 0303 health sciences, biology, Reproduction, food and beverages, Cell cycle, Cullin Proteins, Recombinant Proteins, 3. Good health, Ubiquitin ligase, Cell biology, proteasome gene family, embryonic structures, ox protein, Cullin, Flower morphogenesis, Saccharomyces cerevisiae Proteins, Genetic Vectors, Molecular Sequence Data, Saccharomyces cerevisiae, Spindle Apparatus, Article, 03 medical and health sciences, Transformation, Genetic, cycle-dependent proteolysis, Skp1, thaliana, Amino Acid Sequence, Molecular Biology, Metaphase, 030304 developmental biology, Cell Nucleus, Sequence Homology, Amino Acid, Arabidopsis Proteins, fungi, SCF, Cell Biology, biology.organism_classification, Molecular biology, biology.protein, f-bauxin response, Sequence Alignment, Gene Deletion, 010606 plant biology & botany |
| الوصف: | International audience; The SCF (for SKP1, Cullin/CDC53, F-box protein) ubiquitin ligase targets a number of cell cycle regulators, transcription factors, and other proteins for degradation in yeast and mammalian cells. Recent genetic studies demonstrate that plant F-box proteins are involved in auxin responses, jasmonate signaling, flower morphogenesis, photocontrol. of circadian clocks, and leaf senescence, implying a large spectrum of functions for the SCF pathway in plant development. Here, we present a molecular and functional characterization of plant cullins. The Arabidopsis genome contains 11 cullin-related genes. Complementation assays revealed that AtCUL1 but not AtCUL4 can functionally complement the yeast cdc53 mutant. Arabidopsis mutants containing transfer DNA (T-DNA) insertions in the AtCUL1 gene were shown to display an arrest in early embryogenesis. Consistently, both the transcript and the protein of the AtCUL1 gene were found to accumulate in embryos. The AtCUL1 protein localized mainly in the nucleus but also weakly in the cytoplasm during interphase and colocalized with the mitotic spindle in metaphase. Our results demonstrate a critical role for the SCF ubiquitin ligase in Arabidopsis embryogenesis. |
| تدمد: | 1939-4586 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e2a1ec7e2a756df40c51c050a5a058f https://doi.org/10.1091/mbc.e02-02-0077 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....6e2a1ec7e2a756df40c51c050a5a058f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19394586 10591524 |
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