Hoechst 33258 aggregation and binding to DNA studied by surface-enhanced Raman spectroscopy
| العنوان: | Hoechst 33258 aggregation and binding to DNA studied by surface-enhanced Raman spectroscopy |
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| المؤلفون: | Adriana Kenđel, Virna Zavidić, Snežana Miljanić |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | General Materials Science, Spectroscopy, Hoechst 33258, aggregation, interactions, DNA, SERS |
| الوصف: | Interactions of Hoechst 33258 (H33258) with two synthetic DNA polynucleotides, poly dA – poly dT and poly dG – poly dC, as well as natural calf thymus (ct) DNA were studied using surface- enhanced Raman spectroscopy (SERS). Given variety of stoichiometry dependent interactions, complexes were prepared in [H33258]/[DNA] molar ratios of 1/1, 1/5 and 1/10 in the silver colloidal suspension. Prior to analysis of binding modes with DNA polynucleotides, aggregation tendency of the dye in the silver colloid was studied in the concentration range 1 × 10–6–1 × 10–4 mol/L. The SERS spectra distinctive of aggregated and monomeric H33258 species were obtained, additionally indicating the form of the molecules bound to DNA. The obtained SERS spectra of H33258 with homo-polymeric polynucleotides implied a deep insertion of the molecules into the AT minor groove, and a partial intercalation between the GC base pairs achieved by the access through the GC major groove. Spectral characteristics of the dye dominated in the spectra of the complexes with ct- DNA, most likely due to random AT and GC sequences of the long natural nucleic acid, affecting uniform binding of the dye and consequently the adsorption of the H33258/DNA complex onto the enhancing metallic surface. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::738a2c9d5306b34c6e8fbfd68639b368 https://doi.org/10.1002/jrs.6319 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....738a2c9d5306b34c6e8fbfd68639b368 |
| قاعدة البيانات: | OpenAIRE |
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