Defining Endocytic Pathways of Fucoidan-Coated PIBCA Nanoparticles from the Design of their Surface Architecture
العنوان: | Defining Endocytic Pathways of Fucoidan-Coated PIBCA Nanoparticles from the Design of their Surface Architecture |
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المؤلفون: | M. C. B. Lira-Nogueira, V. P. Gibson, V. Nicolas, N. S. Santos-Magalhães, C. Vauthier |
المساهمون: | Vauthier, Christine, Universidade Federal de Pernambuco [Recife] (UFPE), Ingénierie et Plateformes au Service de l'Innovation Thérapeutique (IPSIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Galien Paris-Saclay (IGPS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
المصدر: | Pharmaceutical Research Pharmaceutical Research, In press, 2022 (Mar 1), pp.1-16. ⟨10.1007/s11095-022-03202-4⟩ |
بيانات النشر: | Springer Science and Business Media LLC, 2022. |
سنة النشر: | 2022 |
مصطلحات موضوعية: | Pharmacology, Organic Chemistry, cellular uptake, Pharmaceutical Science, PIBCA, [SDV.SP.PG] Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, endocytic pathway, [SDV.SP.PG]Life Sciences [q-bio]/Pharmaceutical sciences/Galenic pharmacology, fucoidan, Nanoparticles, Molecular Medicine, Pharmacology (medical), [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, Biotechnology |
الوصف: | International audience; PURPOSE: This work investigated the endocytic pathways taken by poly(isobutylcyanoacrylate) (PIBCA) nanoparticles differing in their surface composition and architecture, assuming that this might determine their efficiency of intracellular drug delivery.METHODS: Nanoparticles (A0, A25, A100, R0, R25) were prepared by anionic or redox radical emulsion polymerization using mixtures of dextran and fucoidan (0, 25, 100 % in fucoidan). Cell uptake was evaluated by incubating J774A.1 macrophages with nanoparticles. Endocytic pathways were studied by incubating cells with endocytic pathway inhibitors (chlorpromazine, genistein, cytochalasin D, methyl-ß-cyclodextrin and nocodazole) and nanoparticle uptake was evaluated by flow cytometry and confocal microscopy. RESULTS: The fucoidan-coated PIBCA nanoparticles A25 were internalized 3-fold more efficiently than R25 due to different architecture of the fucoidan chains presented on the surface. Different fucoidan density and architecture lead to different internalization pathway preferred by the cells. Large A100 nanoparticles which surface was covered with fucoidan chains in a loop and train configuration were internalized the most efficiently, 47-fold compared with A0, and 3-fold compared with R0 and R25 through non-endocytic energy-independent pathways and reached the cell cytoplasm. CONCLUSION: Internalization pathways of PIBCA nanoparticles by J774A.1 macrophages could be determined by nanoparticle fucoidan surface composition and architecture. In turn, this influenced the extent of internalization and localization of accumulated nanoparticles within cells. The results are of interest for rationalizing the design of nanoparticles for potential cytoplamic drug delivery by controlling their pathway of internalization by cells through the nature of the nanoparticle surface |
وصف الملف: | application/pdf |
تدمد: | 1573-904X 0724-8741 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::73f61f6884e672840c84033ae48770b8 https://doi.org/10.1007/s11095-022-03202-4 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....73f61f6884e672840c84033ae48770b8 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 1573904X 07248741 |
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