Suspension culture of human induced pluripotent stem cell-derived intestinal organoids using natural polysaccharides

التفاصيل البيبلوغرافية
العنوان: Suspension culture of human induced pluripotent stem cell-derived intestinal organoids using natural polysaccharides
المؤلفون: Isamu Ogawa, Daichi Onozato, Shiho Anno, Hisato Hayashi, Tatsuro Kanaki, Takahiro Iwao, Tamihide Matsunaga
المصدر: Biomaterials. 288:121696
بيانات النشر: Elsevier BV, 2022.
سنة النشر: 2022
مصطلحات موضوعية: Sulfur Compounds, Induced Pluripotent Stem Cells, Biophysics, Cell Differentiation, Bioengineering, Intestines, Organoids, Biomaterials, Polysaccharides, Mechanics of Materials, Ceramics and Composites, Animals, Humans, Intestinal Mucosa
الوصف: Currently, there are many challenges in the culture of human induced pluripotent stem (iPS) cell-derived intestinal organoids (HIOs) for use in drug discovery, disease research, and regenerative medicine. For example, the main culture method, embedding culture, makes industrial large-scale culture difficult, and Matrigel, which is used for almost all HIO cultures, is not respected for its application in regenerative medicine. To overcome these challenges, we herein propose a new culture method using low concentrations of natural polysaccharides in a suspension culture. In the present study, five natural polysaccharides free from heterologous animal-derived components were used, and HIOs were successfully cultured in suspension with FP001 and FP003, which are microbial exopolysaccharide analogs of gellan gum. The fabricated HIOs were similar to living intestinal tracts with respect to their gene expression, microstructure, and protein expression. The observed activities of the drug metabolizing enzymes and drug transporters in the generated HIOs suggested that they have pharmacokinetic functions. We believe that suspension culture of HIOs using FP001 or FP003 can be widely applied to not only drug discovery research but also disease research and regenerative medicine.
تدمد: 0142-9612
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::768d191100967897e6a52e1fd37fc703
https://doi.org/10.1016/j.biomaterials.2022.121696
حقوق: CLOSED
رقم الأكسشن: edsair.doi.dedup.....768d191100967897e6a52e1fd37fc703
قاعدة البيانات: OpenAIRE