Neutrophil extracellular traps in ulcerative colitis:a proteome analysis of intestinal biopsies
| العنوان: | Neutrophil extracellular traps in ulcerative colitis:a proteome analysis of intestinal biopsies |
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| المؤلفون: | Vibeke Andersen, Svend Birkelund, Henning Glerup, Martin Bøgsted, Thomas Gelsing Carlsen, Ole K. Bonderup, Gunna Christiansen, Tue Bjerg Bennike, Allan Stensballe, Torkell Ellingsen |
| المصدر: | Bennike, T B, Carlsen, T G, Ellingsen, T, Bonderup, O K, Glerup, H, Bøgsted, M, Christiansen, G, Birkelund, S, Stensballe, A & Andersen, V 2015, Neutrophil extracellular traps in ulcerative colitis : a proteome analysis of intestinal biopsies . in Congress Abstract Book, 14th Human Proteome Organization World Congress, HUPO2015, 27-30 September 2015, Vancouver, Canada . Human Proteome Organization, pp. 25, No. CS07.05, Human Proteome Organization World Congress, HUPO, Vancouver, Canada, 27/09/2015 . < http://hupo2015.com/wp-content/uploads/2015/09/HUPO2015-Abstract-Book__vF.pdf > Bennike, T B, Carlsen, T G, Ellingsen, T, Bonderup, O K, Glerup, H, Bøgsted, M, Christiansen, G, Birkelund, S, Stensballe, A & Andersen, V 2015, Neutrophil extracellular traps in ulcerative colitis : A proteome analysis of intestinal biopsies . in Proceedings, Targeted Proteomics Workshop & International Symposium, TPWIS 2015, 10-14 December 2015, Mumbai, India . Indian Institute of Technology, pp. 97-98, No. D-1-07, Targeted Proteomics Workshop & International Symposium 2015, Mumbai, India, 10/12/2015 . Inflammatory Bowel Diseases Bjerg Bennike, T, Carlsen, T G, Ellingsen, T, Bonderup, O K, Glerup, H, Bøgsted, M, Christiansen, G, Birkelund, S, Stensballe, A & Andersen, V 2015, ' Neutrophil Extracellular Traps in Ulcerative Colitis : A Proteome Analysis of Intestinal Biopsies ', Inflammatory Bowel Diseases, vol. 21, no. 9, pp. 2052-2067 . https://doi.org/10.1097/MIB.0000000000000460 Bennike, T B, Carlsen, T G, Ellingsen, T, Bonderup, O K, Glerup, H, Bøgsted, M, Christiansen, G, Birkelund, S, Stensballe, A & Andersen, V 2015, ' Neutrophil extracellular traps in ulcerative colitis : a proteome analysis of intestinal biopsies ', Inflammatory Bowel Diseases, vol. 21, no. 9, pp. 2052-2067 . https://doi.org/10.1097/MIB.0000000000000460 Bennike, T B, Carlsen, T G, Ellingsen, T, Bonderup, O K, Glerup, H, Bøgsted, M, Christiansen, G, Birkelund, S, Stensballe, A & Andersen, V 2015, ' Neutrophil Extracellular Traps in Ulcerative Colitis : A Proteome Analysis of Intestinal Biopsies ', Inflammatory Bowel Diseases, vol. 21, no. 9, pp. 2052-67 . https://doi.org/10.1097/MIB.0000000000000460 |
| بيانات النشر: | Human Proteome Organization, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Proteomics, Extracellular Traps, Pathology, Proteome, Neutrophils, Biopsy, Extracellular Traps/genetics, Neutrophil extracellular traps, Feces, Immunology and Allergy, Intestinal Mucosa, Colitis, Ulcerative/genetics, Microscopy, medicine.diagnostic_test, Gastroenterology, Middle Aged, Ulcerative colitis, Intestines, Lactoferrin/analysis, microscopy, Female, Proteomics/methods, Intestines/immunology, Adult, medicine.medical_specialty, Original Basic Science Articles, neutrophil extracellular traps, Inflammatory bowel diseases, Biology, inflammatory bowel diseases, Feces/chemistry, Proteome/immunology, medicine, Extracellular, Humans, Neutrophils/metabolism, Colitis, ulcerative colitis, Aged, Histology, medicine.disease, Immunity, Innate, Lactoferrin, Case-Control Studies, Immunology, Colitis, Ulcerative, Leukocyte L1 Antigen Complex/analysis, Calprotectin, Leukocyte L1 Antigen Complex, Intestinal Mucosa/metabolism |
| الوصف: | Article first published online 19 May 2015. Background: The etiology of the inflammatory bowel diseases, including ulcerative colitis (UC), remains incompletely explained. We hypothesized that an analysis of the UC colon proteome could reveal novel insights into the disease etiology. Methods: Mucosal colon biopsies were taken by endoscopy from noninflamed tissue of 10 patients with UC and 10 controls. The biopsies were either snap-frozen for protein analysis or prepared for histology. The protein content of the biopsies was characterized by high-throughput gel-free quantitative proteomics, and biopsy histology was analyzed by light microscopy and confocal microscopy. Results: We identified and quantified 5711 different proteins with proteomics. The abundance of the proteins calprotectin and lactotransferrin in the tissue correlated with the degree of tissue inflammation as determined by histology. However, fecal calprotectin did not correlate. Forty-six proteins were measured with a statistically significant differences in abundances between the UC colon tissue and controls. Eleven of the proteins with increased abundances in the UC biopsies were associated with neutrophils and neutrophil extracellular traps. The findings were validated by microscopy, where an increased abundance of neutrophils and the presence of neutrophil extracellular traps by extracellular DNA present in the UC colon tissue were confirmed. Conclusions: Neutrophils, induced neutrophil extracellular traps, and several proteins that play a part in innate immunity are all increased in abundance in the morphologically normal colon mucosa from patients with UC. The increased abundance of these antimicrobial compounds points to the stimulation of the innate immune system in the etiology of UC. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76d20b78dd0befb7a1dde71f13ebd9bb https://vbn.aau.dk/da/publications/5e659613-acf4-4499-b294-efc4b0374664 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....76d20b78dd0befb7a1dde71f13ebd9bb |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |