Indigo Naturalis ameliorates murine dextran sodium sulfate-induced colitis via aryl hydrocarbon receptor activation
| العنوان: | Indigo Naturalis ameliorates murine dextran sodium sulfate-induced colitis via aryl hydrocarbon receptor activation |
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| المؤلفون: | Shinichiro Shinzaki, Manabu Araki, Shoichiro Kawai, Masahiko Tsujii, Eri Shiraishi, Tetsuo Takehara, Daisuke Motooka, Satoshi Hiyama, Akira Mukai, Tetsuya Iida, Toshio Yamaguchi, Takahiro Inoue, Shuko Iwatani, Hideki Iijima, Yoshito Hayashi, Shota Nakamura |
| المصدر: | Journal of Gastroenterology. 52:904-919 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, 0301 basic medicine, medicine.medical_specialty, CD3 Complex, T-Lymphocytes, medicine.medical_treatment, Gene Expression, Pharmacology, Indigo Carmine, Severity of Illness Index, T-Lymphocytes, Regulatory, Inflammatory bowel disease, Indigo, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, medicine, Animals, RNA, Messenger, Intestinal Mucosa, Colitis, Cells, Cultured, Mice, Knockout, biology, Chemistry, Interleukins, Dextran Sulfate, Interleukin-2 Receptor alpha Subunit, Gastroenterology, Interleukin, Forkhead Transcription Factors, Nuclear Receptor Subfamily 1, Group F, Member 3, medicine.disease, Aryl hydrocarbon receptor, CD4 Lymphocyte Count, Interleukin-10, Interleukin 10, 030104 developmental biology, Endocrinology, Cytokine, Receptors, Aryl Hydrocarbon, Trinitrobenzenesulfonic Acid, Indigo carmine, Leukocytes, Mononuclear, biology.protein, Female, Spleen, Drugs, Chinese Herbal |
| الوصف: | Indigo Naturalis (IN) is used as a traditional herbal medicine for ulcerative colitis (UC). However, the mechanisms of action of IN have not been clarified. We aimed to evaluate the efficacy of IN for ameliorating colonic inflammation. We further investigated the mechanisms of action of IN. Colitis severity was assessed in dextran sodium sulfate-induced colitis and trinitrobenzene sulfonic acid-induced colitis models with or without the oral administration of IN or indigo, which is a known major component of IN. Colonic lamina propria (LP) mononuclear cells isolated from IN-treated mice were analyzed with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and flow cytometry. LP and splenic mononuclear cells cultured in vitro with IN or indigo were also analyzed. The role of the candidate receptor for indigo, the aryl hydrocarbon receptor (AhR), was analyzed using Ahr-deficient mice. Colitis severity was significantly ameliorated in the IN and indigo treatment groups compared with the control group. The mRNA expression levels of interleukin (Il)-10 and Il-22 in the LP lymphocytes were increased by IN treatment. The treatment of splenocytes with IN or indigo increased the expression of anti-inflammatory cytokines and resulted in the expansion of IL-10-producing CD4+ T cells and IL-22-producing CD3−RORγt+ cells, but not CD4+Foxp3+ regulatory T cells. The amelioration of colitis by IN or indigo was abrogated in Ahr-deficient mice, in association with diminished regulatory cytokine production. IN and indigo ameliorated murine colitis through AhR signaling activation, suggesting that AhR could be a promising therapeutic target for UC. |
| تدمد: | 1435-5922 0944-1174 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::782ba7e5501b6ebab920dff68ce48d5c https://doi.org/10.1007/s00535-016-1292-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....782ba7e5501b6ebab920dff68ce48d5c |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14355922 09441174 |
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