Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model
| العنوان: | Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model |
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| المؤلفون: | Han-Jun Kim, Sun Hee Do, Hye-Rim Lee, Sukyoung Kim, Oog-Jin Shon, Se-Il Park, Myun-Whan Ahn |
| المصدر: | International Journal of Molecular Sciences, Vol 17, Iss 1, p 120 (2016) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(17): 1 International Journal of Molecular Sciences; Volume 17; Issue 1; Pages: 120 International Journal of Molecular Sciences |
| بيانات النشر: | MDPI AG, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, collagen, animal diseases, Matrix metalloproteinase, Menisci, Tibial, lcsh:Chemistry, 0302 clinical medicine, Fibrosis, 030212 general & internal medicine, Aggrecans, lcsh:QH301-705.5, Spectroscopy, Chemistry, General Medicine, Computer Science Applications, medicine.anatomical_structure, Matrix Metalloproteinase 3, Rabbits, MMPs, medicine.symptom, Matrix Metalloproteinase 1, Type I collagen, platelet-rich plasma, meniscal cells, proteoglycan, Inflammation, Catalysis, Article, Inorganic Chemistry, 03 medical and health sciences, Chondrocytes, In vivo, medicine, Animals, Physical and Theoretical Chemistry, Molecular Biology, Aggrecan, Cartilage, Interleukins, Organic Chemistry, Cell Dedifferentiation, medicine.disease, Molecular biology, nervous system diseases, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Platelet-rich plasma, Immunology |
| الوصف: | Despite the susceptibility to frequent intrinsic and extrinsic injuries, especially in the inner zone, the meniscus does not heal spontaneously owing to its poor vascularity. In this study, the effect of platelet-rich plasma (PRP), containing various growth factors, on meniscal mechanisms was examined under normal and post-traumatic inflammatory conditions. Isolated primary meniscal cells of New Zealand white (NZW) rabbits were incubated for 3, 10, 14 and 21 days with PRP(-), 10% PRP (PRP(+)), IL(+) or IL(+) PRP(+). The meniscal cells were collected and examined using reverse-transcription polymerase chain reaction (RT-PCR). Culture media were examined by immunoblot analyses for matrix metalloproteinases (MMP) catabolic molecules. PRP containing growth factors improved the cellular viability of meniscal cells in a concentration-dependent manner at Days 1, 4 and 7. However, based on RT-PCR, meniscal cells demonstrated dedifferentiation, along with an increase in type I collagen in the PRP(+) and in IL(+) PRP(+). In PRP(+), the aggrecan expression levels were lower than in the PRP(-) until Day 21. The protein levels of MMP-1 and MMP-3 were higher in each PRP group, i.e., PRP(+) and IL(+) PRP(+), at each culture time. A reproducible 2-mm circular defect on the meniscus of NZW rabbit was used to implant fibrin glue (control) or PRP in vivo. After eight weeks, the lesions in the control and PRP groups were occupied with fibrous tissue, but not with meniscal cells. This study shows that PRP treatment of the meniscus results in an increase of catabolic molecules, especially those related to IL-1 alpha-induced inflammation, and that PRP treatment for an in vivo meniscus injury accelerates fibrosis, instead of meniscal cartilage. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a83b48cb057300ecfb837ccbcbd0948 http://www.mdpi.com/1422-0067/17/1/120 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7a83b48cb057300ecfb837ccbcbd0948 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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