Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples
| العنوان: | Optimization of tris(2-carboxyethyl) phosphine reduction conditions for fast analysis of total biothiols in mouse serum samples |
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| المؤلفون: | Muneki Isokawa, Chunfang Chang, Takashi Funatsu, Makoto Tsunoda |
| المصدر: | Heliyon, Vol 5, Iss 5, Pp e01598-(2019) Heliyon |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Tris, Multidisciplinary, Chromatography, Cystine, Glutathione, Article, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, 0302 clinical medicine, chemistry, Reagent, TCEP, Glutathione disulfide, lcsh:H1-99, lcsh:Social sciences (General), lcsh:Science (General), Pharmaceutical chemistry, Analytical chemistry, 030217 neurology & neurosurgery, Phosphine, Cysteine, lcsh:Q1-390 |
| الوصف: | In this study, we investigated suitable conditions for the reduction of disulfides in mouse serum samples by tris(2-carboxyethyl) phosphine (TCEP) for fast analysis of total biothiols. Disulfides were reduced with TCEP, and then, thiols were derivatized with the fluorogenic reagent, ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Interference peaks on chromatograms of mouse serum samples disappeared when the TCEP reaction was conducted on ice instead of at room temperature, which is used classically. Low-molecular-weight disulfides, such as cystine and glutathione disulfide, were nearly completely reduced by TCEP on ice. Six SBD-biothiols (homocysteine, cysteine, cysteinylglycine, glutathione, γ-glutamylcysteine, and N-acetylcysteine) were separated within 7.5 min on a sulfoalkylbetain-type column (ZIC-HILIC: 150 × 2.1 mm i.d., 3.5 μm), without interference peaks. The developed method showed good linearity and reproducibility, with inter- and intra-day precisions of less than 3%. |
| تدمد: | 2405-8440 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bed7e0c1f1217c00c00ac2ddc6de595 https://pubmed.ncbi.nlm.nih.gov/31193090 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7bed7e0c1f1217c00c00ac2ddc6de595 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24058440 |
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