Valyl-tRNA Synthetase from Artemia. Purification and Association with Elongation Factor 1
| العنوان: | Valyl-tRNA Synthetase from Artemia. Purification and Association with Elongation Factor 1 |
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| المؤلفون: | Jan Dijk, George M.C. Janssen, Margreet Brandsma, Wim Möller, Pierre Kerjan |
| المصدر: | European Journal of Biochemistry. 233:277-282 |
| بيانات النشر: | Wiley, 1995. |
| سنة النشر: | 1995 |
| مصطلحات موضوعية: | Valine-tRNA Ligase, Molecular Sequence Data, Brine shrimp, Biology, Biochemistry, chemistry.chemical_compound, Peptide Elongation Factor 1, Protein biosynthesis, Animals, Amino Acid Sequence, Cloning, Molecular, chemistry.chemical_classification, Sequence Homology, Amino Acid, Aminoacyl tRNA synthetase, Immunochemistry, RNA, Peptide Elongation Factors, biology.organism_classification, Molecular biology, Yeast, Eukaryotic translation elongation factor 1 alpha 1, Amino acid, Molecular Weight, Elongation factor, chemistry, Artemia |
| الوصف: | Two components of the protein biosynthetic machinery, valyl-transfer RNA synthetase (VRS) and elongation factor 1 (EF-1), have been isolated as a complex from several mammalian tissues. However, yeast VRS, which lacks an amino-terminal extension, does not associated with EF-1. We purified VRS from the brine shrimp Artemia and investigated its interaction with EF-1. Western blotting of crude Artemia extracts revealed the presence of two forms of VRS, differing in size and capacity to associate with EF-1. About 80% of the total VRS corresponds to a polypeptide of 130 kDa which behaves as a monomer upon gel filtration. Only the larger form of 140 kDa coelutes, cosediments and co-immunoprecipitates with the EF-1 alpha 2 beta gamma delta complex. The ratio of the two forms of VRS remains constant throughout early development. The possible origin and mode of expression of the two forms of VRS present in Artemia are discussed. |
| تدمد: | 1432-1033 0014-2956 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e27f842f74b215462cd932275e08a35 https://doi.org/10.1111/j.1432-1033.1995.277_1.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....7e27f842f74b215462cd932275e08a35 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321033 00142956 |
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