Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission
| العنوان: | Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission |
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| المؤلفون: | Lijian Hui, Xiaoping He, Tong Zhang, Lin Wang, Mian Wu, Yongping Cai, Ping Gao, X.C. Zhong, Lan Hu, Shengya Tian, Lihua Wang, Huafeng Zhang |
| المصدر: | The EMBO Journal. 36:1330-1347 |
| بيانات النشر: | EMBO, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Pluripotent Stem Cells, 0301 basic medicine, FIS1, Somatic cell, Biology, Mitochondrial Dynamics, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, Acetyl Coenzyme A, Humans, Induced pluripotent stem cell, Molecular Biology, Fatty acid synthesis, General Immunology and Microbiology, General Neuroscience, Fatty Acids, Articles, Embryonic stem cell, 030104 developmental biology, Biochemistry, chemistry, Mitochondrial fission, Stem cell, Reprogramming, Acetyl-CoA Carboxylase |
| الوصف: | Pluripotent stem cells are known to display distinct metabolic phenotypes than their somatic counterparts. While accumulating studies are focused on the roles of glucose and amino acid metabolism in facilitating pluripotency, little is known regarding the role of lipid metabolism in regulation of stem cell activities. Here, we show that fatty acid (FA) synthesis activation is critical for stem cell pluripotency. Our initial observations demonstrated enhanced lipogenesis in pluripotent cells and during cellular reprogramming. Further analysis indicated that de novo FA synthesis controls cellular reprogramming and embryonic stem cell pluripotency through mitochondrial fission. Mechanistically, we found that de novo FA synthesis regulated by the lipogenic enzyme ACC1 leads to the enhanced mitochondrial fission via (i) consumption of AcCoA which affects acetylation‐mediated FIS1 ubiquitin–proteasome degradation and (ii) generation of lipid products that drive the mitochondrial dynamic equilibrium toward fission. Moreover, we demonstrated that the effect of Acc1 on cellular reprogramming via mitochondrial fission also exists in human iPSC induction. In summary, our study reveals a critical involvement of the FA synthesis pathway in promoting ESC pluripotency and iPSC formation via regulating mitochondrial fission. |
| تدمد: | 1460-2075 0261-4189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e366df103c4cc1b5e8efe5ad4623247 https://doi.org/10.15252/embj.201695417 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....7e366df103c4cc1b5e8efe5ad4623247 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602075 02614189 |
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