A novel ‘sort-suicide’ fusion gene vector for T cell manipulation
| العنوان: | A novel ‘sort-suicide’ fusion gene vector for T cell manipulation |
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| المؤلفون: | W. Bohn, Boris Fehse, Zhixiong Li, W R Beyer, Klaus Kühlcke, Axel R. Zander, Christopher Baum, O S Kustikova, Pierre Tiberghien, D Chalmers, A Wahlers |
| المصدر: | Gene Therapy. 9:1633-1638 |
| بيانات النشر: | Springer Science and Business Media LLC, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | Genetic Markers, T-Lymphocytes, Genetic enhancement, T cell, Genetic Vectors, Antigens, CD34, Biology, Immunotherapy, Adoptive, Lymphocyte Depletion, Virus, Jurkat Cells, Transduction, Genetic, Genetics, medicine, Humans, Vector (molecular biology), Ganciclovir, Molecular Biology, Reporter gene, Microscopy, Confocal, Hybrid vector, Suicide gene, Virology, Retroviridae, medicine.anatomical_structure, Thymidine kinase, Molecular Medicine |
| الوصف: | Retroviral suicide gene vectors have successfully been used in clinical studies to improve the safety of adoptive immunotherapy with allogeneic T lymphocytes in the treatment of malignant and viral diseases. At the same time these studies have revealed several problems that are yet to be resolved including impaired T cell function due to long ex vivo culture. Here we present new retroviral vectors co-expressing truncated CD34, a gene transfer marker which ensures rapid enrichment of transduced cells using commercially available GMP-approved devices, and a splice-corrected variant of Herpes simplex virus thymidine kinase (scHSVtk) which confers high sensitivity to the prodrug ganciclovir. We show that a retroviral hybrid vector, MP71, based on the myeloproliferative sarcoma virus (MPSV) and the murine embryonic stem cell virus (MESV), encoding a tCD34/scHSVtk fusion protein mediates high expression of the 'sort-suicide' selection marker, thereby allowing for highly efficient purification and selective elimination of transduced cells. |
| تدمد: | 1476-5462 0969-7128 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8028011072a17ad30cb5ad888a0b0311 https://doi.org/10.1038/sj.gt.3301828 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....8028011072a17ad30cb5ad888a0b0311 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765462 09697128 |
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