CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells
| العنوان: | CRISPR-based engineering of gene knockout cells by homology-directed insertion in polyploid Drosophila S2R+ cells |
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| المؤلفون: | Jonathan Zirin, Baolong Xia, Stephanie E. Mohr, Gabriel Amador, Raghuvir Viswanatha, Norbert Perrimon |
| المصدر: | Nat Protoc |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Genomics, Biology, Genome, Article, General Biochemistry, Genetics and Molecular Biology, Homology (biology), DNA sequencing, Polyploidy, Gene Knockout Techniques, 03 medical and health sciences, 0302 clinical medicine, Gene duplication, Animals, CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats, Gene, Alleles, Gene knockout, 030304 developmental biology, Gene Editing, Genetics, 0303 health sciences, Base Sequence, Homozygote, fungi, food and beverages, Endonucleases, Drosophila, CRISPR-Cas Systems, 030217 neurology & neurosurgery, RNA, Guide, Kinetoplastida |
| الوصف: | Precise and efficient genome modifications provide powerful tools for biological studies. Previous CRISPR gene knockout methods in cell lines have relied on frameshifts caused by stochastic insertion/deletion in all alleles. However, this method is inefficient for genes with high copy number due to polyploidy or gene amplification because frameshifts in all alleles can be difficult to generate and detect. Here we describe a homology-directed insertion method to knockout genes in the polyploid Drosophila S2R+ cell line. This protocol allows generation of homozygous mutant cell lines using an insertion cassette which autocatalytically generates insertion mutations in all alleles. Knockout cells generated using this method can be directly identified by PCR without a need for DNA sequencing. This protocol takes 2–3 months and can be applied to other polyploid cell lines or high-copy-number genes. This homology-directed insertion-based CRISPR gene-editing protocol enables knockout of all alleles of a target gene in the polyploid Drosophila S2R+ cell line, using either two sequential rounds of homology-directed insertion or a single round with a donor vector containing four different sgRNAs. |
| تدمد: | 1750-2799 1754-2189 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80a8fad09e0121e2507c0b4dce0677b6 https://doi.org/10.1038/s41596-020-0383-8 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....80a8fad09e0121e2507c0b4dce0677b6 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 17502799 17542189 |
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