Low amounts of heavy water increase the phase separation propensity of a fragment of the androgen receptor activation domain
| العنوان: | Low amounts of heavy water increase the phase separation propensity of a fragment of the androgen receptor activation domain |
|---|---|
| المؤلفون: | Elzbieta Szulc, Stasė Bielskutė, Mireia Pesarrodona, Marta Frigolé-Vivas, Carla Garcia-Cabau, Xavier Salvatella, Jesús García, Eva De Mol |
| المصدر: | Protein Sci Dipòsit Digital de la UB Universidad de Barcelona |
| بيانات النشر: | John Wiley & Sons, Inc., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | inorganic chemicals, Phase transition, Full‐Length Papers, Hormone receptors, Intrinsically disordered proteins, Biochemistry, Hydrophobic effect, 03 medical and health sciences, Protein Domains, Phase (matter), Humans, Deuterium Oxide, Molecular Biology, Transcription factor, 030304 developmental biology, 0303 health sciences, Receptors d'hormones, Chemistry, 030302 biochemistry & molecular biology, Condensation, Proteins, In vitro, Androgen receptor, Intrinsically Disordered Proteins, Receptors, Androgen, Biophysics, Proteïnes |
| الوصف: | The phase equilibria of intrinsically disordered proteins are exquisitely sensitive to changes in solution conditions and this can be used to investigate the driving forces of phase separation in vitro as well as the biological roles of phase transitions in live cells. Here we investigate how using D(2)O as co‐solvent in an aqueous buffer changes the phase equilibrium of a fragment of the activation domain of the androgen receptor, a transcription factor that plays a role in the development of the male phenotype and is a therapeutic target for castration resistant prostate cancer. We show how replacing even small fractions of H(2)O with D(2)O increases the propensity of this fragment to undergo liquid–liquid phase separation, likely reflecting a stabilization of the hydrophobic interactions that drive condensation. Our results indicate that it is necessary to take this effect into consideration when studying phase separation phenomena with biophysical methods that require using D(2)O as a co‐solvent. In addition, they suggest that additions of D(2)O may be used to enhance phase separation phenomena in cells, facilitating their observation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81172ba538b61b1ea0f3c3640b3291db https://europepmc.org/articles/PMC8197436/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....81172ba538b61b1ea0f3c3640b3291db |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |