Clinical heterogeneity of patients with stool samples testing PCR+/Tox− from a two-step Clostridium difficile diagnostic algorithm
| العنوان: | Clinical heterogeneity of patients with stool samples testing PCR+/Tox− from a two-step Clostridium difficile diagnostic algorithm |
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| المؤلفون: | Trong Tien Nguyen, Elisa Lloyd-Smith, Michael Payne, Azra Sharma, Sylvie Champagne, Christopher F. Lowe, Jason Zou, Anna Wong, Victor C. M. Leung, Marc G. Romney, Michelle Hinch |
| المصدر: | European Journal of Clinical Microbiology & Infectious Diseases. 37:2355-2359 |
| بيانات النشر: | Springer Science and Business Media LLC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Diarrhea, 0301 basic medicine, Microbiology (medical), Canada, medicine.medical_specialty, Bacterial Toxins, 030106 microbiology, Two step, Clostridium difficile toxin A, Polymerase Chain Reaction, Enterotoxins, Feces, 03 medical and health sciences, 0302 clinical medicine, Medical microbiology, Bacterial Proteins, medicine, Humans, Antimicrobial stewardship, Clinical significance, 030212 general & internal medicine, Enterocolitis, Pseudomembranous, Retrospective Studies, medicine.diagnostic_test, Clostridioides difficile, business.industry, General Medicine, Clostridium difficile, Hospitals, Patient Outcome Assessment, Infectious Diseases, Immunoassay, Clostridium Infections, medicine.symptom, business, Algorithm, Algorithms |
| الوصف: | The clinical significance of indeterminate (PCR+/Tox-) results for patients tested with a two-step algorithm for Clostridium difficile infection (CDI) is uncertain. We aimed to evaluate the clinical presentation and 8-week outcomes of patients with indeterminate test results. Patients with stool samples testing positive by PCR and negative by toxin A/B immunoassay between February 1, 2017, and April 30, 2018, were assessed by antimicrobial stewardship program (ASP) clinicians and classified as colonized or infected. Retrospective chart review was performed to obtain outcomes occurring within 8 weeks of testing, including recurrent C. difficile diarrhea, subsequent treatment for CDI, follow-up C. difficile testing, all-cause mortality, and CDI-related complications. In total, 110 PCR+/Tox- patients were evaluated. ASP classified 54% of patients as infected and 46% as colonized. Patients assessed and classified as colonized did not have increased adverse outcomes by 8 weeks compared to those assessed as infected, despite not receiving treatment for CDI. We conclude that PCR+/Tox- patients are heterogeneous with respect to clinical presentation. Negative toxin A/B immunoassay in a two-step algorithm should not be interpreted in isolation to distinguish colonization from infection as many PCR+/Tox- results may be clinically significant for CDI. |
| تدمد: | 1435-4373 0934-9723 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81e87c5da906fa4b0e444e30762a3031 https://doi.org/10.1007/s10096-018-3383-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....81e87c5da906fa4b0e444e30762a3031 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14354373 09349723 |
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