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Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa

التفاصيل البيبلوغرافية
العنوان: Isolation and characterization of a lytic bacteriophage against Pseudomonas aeruginosa
المؤلفون: Moumita Dutta, Rajeev Gupta, Sanjai K. Dwivedi, Mohan G. Vairale, Sonika Sharma, Soumya Chatterjee, Sibnarayan Datta, Jhuma Samanta, Vijay Veer
المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-22 (2021)
Scientific Reports
بيانات النشر: Nature Portfolio, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Science, Genome, Viral, Wastewater, medicine.disease_cause, Microbiology, Genome, behavioral disciplines and activities, Article, Homology (biology), Bacteriophage, Environmental biotechnology, Drug Resistance, Multiple, Bacterial, medicine, Sequencing, Bacteriophages, ORFS, Multidisciplinary, biology, Pseudomonas aeruginosa, Toxin, musculoskeletal, neural, and ocular physiology, biology.organism_classification, Lytic cycle, Myoviridae, DNA, Viral, Medicine, Pseudomonas Phages, Bacteria, psychological phenomena and processes, Biotechnology
الوصف: In recent years, the use of bacteriophages (or 'phages') against multidrug-resistant (MDR) bacteria including Pseudomonas aeruginosa has drawn considerable attention, globally. In this work, we report the isolation and detailed characterization of a highly lytic Pseudomonasphage DRL-P1 isolated from wastewater. Under TEM, DRL-P1 appeared as a member of the phage family Myoviridae. DRL-P1 featured rapid adsorption (~ 5 min), short-latency (~ 30 min), and large burst size (~ 100 PFU per infected cell). DRL-P1 can withstand a wide temperature range (4 °C to 40 °C) and pH (5.0 to 10.0) conditions. The 66,243 bp DRL-P1 genome (MN564818) encodes at least 93 ORFs, of which 36 were functionally annotated based on homology with similar phage proteins available in the databases. Comparative analyses of related genomes suggest an independent evolutionary history and discrete taxonomic position of DRL-P1 within genus Pbunavirus. No toxin or antibiotic resistance genes was identified. DRL-P1 is tolerant to lyophilization and encapsulation techniques and retained lytic activity even after 18 months of storage. We also demonstrated decontaminating potentials of DRL-P1 in vitro, on an artificially contaminated cover-slip model. To the best of our knowledge, this is the first Pbunavirus to be reported from India. Our study suggests DRL-P1 as a potential candidate for various applications.
اللغة: English
تدمد: 2045-2322
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8235ba0cc97fe1dd6d65e0cf68104b61
https://doaj.org/article/cd12c250461c4d71b16dadc0bc7f84d5
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....8235ba0cc97fe1dd6d65e0cf68104b61
قاعدة البيانات: OpenAIRE
الوصف
تدمد:20452322