Adipose tissue mitochondrial dysfunction in human obesity is linked to a specific DNA methylation signature in adipose-derived stem cells
| العنوان: | Adipose tissue mitochondrial dysfunction in human obesity is linked to a specific DNA methylation signature in adipose-derived stem cells |
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| المؤلفون: | Jeffrey M. Gimble, Pablo M. Garcia-Roves, Catalina Núñez-Roa, Xevi Duran, Victoria Ceperuelo-Mallafré, Miquel A. Peinado, Pau Gama, Elsa Maymó-Masip, Antonio Zorzano, Monica Millan-Scheiding, Angels Díaz-Ramos, Miriam Ejarque, Carolina Serena, Sonia Fernández-Veledo, Joan Vendrell, Yaiza Núñez-Álvarez |
| المصدر: | International Journal of Obesity r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname Dipòsit Digital de la UB Universidad de Barcelona International Journal of Obesity (2005) |
| بيانات النشر: | Nature Publishing Group, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, medicine.medical_specialty, Endocrinology, Diabetes and Metabolism, Population, Medicine (miscellaneous), Adipose tissue, 030209 endocrinology & metabolism, Mitochondrion, Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Thinness, Internal medicine, Adipocyte, Adipocytes, medicine, Humans, Obesity, 030212 general & internal medicine, education, Human obesity, Inflammation, education.field_of_study, Adipogenesis, Cèl·lules animals, Nutrition and Dietetics, Stem Cells, Adipose tissues, DNA Methylation, Translational research, Lipids, Cell biology, Mitochondria, Oxidative Stress, Teixit adipós, Endocrinology, Adipose Tissue, Animal cells, Lipotoxicity, chemistry, Lípids, DNA methylation, Female, Stem cell |
| الوصف: | Background A functional population of adipocyte precursors, termed adipose-derived stromal/stem cells (ASCs), is crucial for proper adipose tissue (AT) expansion, lipid handling, and prevention of lipotoxicity in response to chronic positive energy balance. We previously showed that obese human subjects contain a dysfunctional pool of ASCs. Elucidation of the mechanisms underlying abnormal ASC function might lead to therapeutic interventions for prevention of lipotoxicity by improving the adipogenic capacity of ASCs. Methods Using epigenome-wide association studies, we explored the impact of obesity on the methylation signature of human ASCs and their differentiated counterparts. Mitochondrial phenotyping of lean and obese ASCs was performed. TBX15 loss- and gain-of-function experiments were carried out and western blotting and electron microscopy studies of mitochondria were performed in white AT biopsies from lean and obese individuals. Results We found that DNA methylation in adipocyte precursors is significantly modified by the obese environment, and adipogenesis, inflammation, and immunosuppression were the most affected pathways. Also, we identified TBX15 as one of the most differentially hypomethylated genes in obese ASCs, and genetic experiments revealed that TBX15 is a regulator of mitochondrial mass in obese adipocytes. Accordingly, morphological analysis of AT from obese subjects showed an alteration of the mitochondrial network, with changes in mitochondrial shape and number. Conclusions We identified a DNA methylation signature in adipocyte precursors associated with obesity, which has a significant impact on the metabolic phenotype of mature adipocytes. |
| وصف الملف: | application/pdf |
| تدمد: | 0307-0565 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84a7bb62342b24d4f3ce8b10b718a774 https://fundanet.igtp.cat/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2926 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....84a7bb62342b24d4f3ce8b10b718a774 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 03070565 |
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