Common Amino Acid Sequences Deduced from Coding Exons of the PorcineFGF4Gene in Two Breeds and Production of the Encoded Protein inEscherichia coli
| العنوان: | Common Amino Acid Sequences Deduced from Coding Exons of the PorcineFGF4Gene in Two Breeds and Production of the Encoded Protein inEscherichia coli |
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| المؤلفون: | Toshihiko Ito, Toshikiyo Takahashi, Jun-ichi Konishi, Kano Kasuga, Tomokazu Fukuda, Saiko Sugawara, Masaki Kobayashi, Ikuo Kojima, Hitoshi Suzuki, Sho Sato, Masayuki Kobayashi, Yuki Mori |
| المصدر: | Bioscience, Biotechnology, and Biochemistry. 77:173-177 |
| بيانات النشر: | Oxford University Press (OUP), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Swine, Recombinant Fusion Proteins, Molecular Sequence Data, Fibroblast Growth Factor 4, Sequence alignment, Breeding, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Biochemistry, Analytical Chemistry, Open Reading Frames, FGF4, Escherichia coli, medicine, Animals, Cloning, Molecular, Molecular Biology, Gene, FGF10, Base Sequence, Organic Chemistry, Structural gene, Nucleic acid sequence, Exons, General Medicine, Fibroblasts, Molecular biology, Open reading frame, Sequence Alignment, Biotechnology |
| الوصف: | Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the development of mammalian embryos. Here we identified common amino acid sequences predicted from coding exons of the FGF4 gene in five pigs of two breeds, and HispFGF4, a 6× histidine-tagged porcine FGF4, was produced in Escherichia coli. HispFGF4 was purified efficiently from the supernatant of cell lysate by heparin column chromatography. In a porcine embryonic fibroblast cell line, HispFGF4 showed significant mitogenic activities at concentrations as low as 0.001 nM (p |
| تدمد: | 1347-6947 0916-8451 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b37bb2c437f7ce8f42e3ab9695e822 https://doi.org/10.1271/bbb.120698 |
| رقم الأكسشن: | edsair.doi.dedup.....86b37bb2c437f7ce8f42e3ab9695e822 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 13476947 09168451 |
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