Direct effects of casein phosphopeptides on growth and differentiation of in vitro cultured osteoblastic cells (MC3T3-E1)
| العنوان: | Direct effects of casein phosphopeptides on growth and differentiation of in vitro cultured osteoblastic cells (MC3T3-E1) |
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| المؤلفون: | Daniela Cocchi, Giovanni Tulipano, Anna Maria Caroli, Alessandro Nardone, Omar Bulgari, Stefania Chessa |
| المصدر: | Regulatory peptides 160 (2010): 168–174. doi:10.1016/j.regpep.2009.11.018 info:cnr-pdr/source/autori:Giovanni Tulipano; Omar Bulgari; Stefania Chessa; Alessandro Nardone; Daniela Cocchi; Anna Caroli/titolo:Direct effects of casein phosphopeptides on growth and differentiation of in vitro cultured osteoblastic cells (MC3T3-E1)/doi:10.1016%2Fj.regpep.2009.11.018/rivista:Regulatory peptides (Print)/anno:2010/pagina_da:168/pagina_a:174/intervallo_pagine:168–174/volume:160 |
| بيانات النشر: | Elsevier BV, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Phosphopeptides, Mineralization, Cell Survival, Physiology, Cellular differentiation, Molecular Sequence Data, Clinical Biochemistry, chemistry.chemical_element, Cell Communication, Calcium, Biology, Biochemistry, Extracellular matrix, Mice, Cellular and Molecular Neuroscience, Endocrinology, Milk protein variants, Casein, MC3T3-E1 osteoblasts, Casein phosphopeptides, Bone cell, medicine, Animals, Amino Acid Sequence, Cells, Cultured, chemistry.chemical_classification, MC3T3-E1 osteoblasts, Casein phosphopeptides, Milk protein variants, Mineralization, Osteoblasts, Caseins, Cell Differentiation, Osteoblast, Alkaline Phosphatase, In vitro, Amino acid, Enzyme Activation, medicine.anatomical_structure, chemistry |
| الوصف: | Casein phosphopeptides (CPPs) obtained by enzymatic hydrolysis in vitro of caseins, have been shown to enhance calcium solubility and to increase the calcification of embryonic rat bones in their diaphyseal area. Little is known about the direct effects of CPPs on cultured osteoblastic cells. Calcium in the microenvironment surrounding bone cells is not only important for the mineralization of the extracellular matrix, but it is believed to provide preosteblasts with a signal that modulates their proliferation and differentiation. The aim of the present study was to investigate the direct effects of four selected casein phosphopeptides on osteoblastic cell (MC3T3-E1 cells) viability and differentiation. The selected peptides have been obtained by chemical synthesis and differed in the number of phosphorylated sites and in the amino acid spacing out two phosphorylated sites, in order to further characterize the relationship between structure and function. The results obtained in this work demonstrated that CPPs may directly affect osteoblast-like cell growth, calcium uptake and ultimately calcium deposition in the extracellular matrix. The effects exerted by distinct CPPs on osteogenesis in vitro can be either stimulatory or inhibitory. Differential short amino acid sequences in their molecules, like the -SpEE- and the -SpTSpEE-motifs, are likely the molecular determinants for their biological activities on osteoblastic cells. Moreover, two genetic variants of CPPs showing one amino acid change in their sequence may profoundly differ in their biological activities. Finally, our data may also suggest important clues about the role of intrinsic phosphorylated peptides derived from endogenous phosphorylated proteins in bone metabolism, apart from extrinsic CPPs. |
| تدمد: | 0167-0115 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88f0a97bc0f941b3d1f1c846d45baedd https://doi.org/10.1016/j.regpep.2009.11.018 |
| حقوق: | RESTRICTED |
| رقم الأكسشن: | edsair.doi.dedup.....88f0a97bc0f941b3d1f1c846d45baedd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 01670115 |
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