Immobilisation of an anti-platelet adhesion and anti-thrombotic drug (EP224283) on polydopamine coated vascular stent promoting anti-thrombogenic properties
| العنوان: | Immobilisation of an anti-platelet adhesion and anti-thrombotic drug (EP224283) on polydopamine coated vascular stent promoting anti-thrombogenic properties |
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| المؤلفون: | Adrien Hertault, Nicolas Bricout, Feng Chai, Nicolas Blanchemain, Jonathan Sobocinski, Joel Lyskawa, Alexandre Ung, William Laure, Patrice Woisel |
| المساهمون: | Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 (MBLC - ADDS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 (RNMCD), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM), French Society of Radiology SFR2014OSEO A11016013N, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), CCSD, Accord Elsevier |
| المصدر: | Materials Science and Engineering: C Materials Science and Engineering: C, 2020, Materials Science and Engineering: C, pp.110967. ⟨10.1016/j.msec.2020.110967⟩ |
| بيانات النشر: | HAL CCSD, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Chromium, Polydopamine, Indoles, medicine.drug_mechanism_of_action, Polymers, medicine.medical_treatment, Idraparinux, Oligosaccharides, Biocompatible Materials, 02 engineering and technology, [SPI.MAT] Engineering Sciences [physics]/Materials, 01 natural sciences, [SPI.MAT]Engineering Sciences [physics]/Materials, chemistry.chemical_compound, Surface functionalisation, Biotin, Restenosis, Stent, Polyethyleneimine, biology, Platelet adhesion, Drug-Eluting Stents, Cobalt, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, EP224283, Mechanics of Materials, Factor Xa, 0210 nano-technology, [CHIM.POLY] Chemical Sciences/Polymers, Materials science, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, Cell Survival, Surface Properties, Factor Xa Inhibitor, Bioengineering, 010402 general chemistry, Biomaterials, Platelet Adhesiveness, Human Umbilical Vein Endothelial Cells, medicine, Humans, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, Platelet activation, [CHIM.MATE] Chemical Sciences/Material chemistry, Polyethylenimine, Thrombosis, Avidin, Platelet Activation, medicine.disease, 0104 chemical sciences, [CHIM.POLY]Chemical Sciences/Polymers, chemistry, biology.protein, Biomedical engineering |
| الوصف: | International audience; Current vascular drug-eluting stents based on immuno-proliferative drugs would reduce the rate of in-stent restenosis (ISR) but may be associated with a higher risk of acute stent thrombosis due to non-selective activity. In this paper, we aimed to develop a polydopamine (PDA) coated chromium‑cobalt (CoCr) stent functionalised with EP224283 (Endotis Pharma SA), which combines both a GPIIbIIIa antagonist (tirofiban moiety) and a factor Xa inhibitor (idraparinux moiety) to reduce acute stent thrombosis.PDA-coated chromium‑cobalt (CoCr) samples were first immersed in a polyethylenimine (PEI, pH 8.5) solution to increase amine function density (36.0 ± 0.1 nmol/cm2) on the CoCr surface. In a second step, avidin was grafted onto CoCr-PDA-PEI through the biotin linkage (strategy 1) or directly by coupling reactions (strategy 2). The HABA titration proved the fixation of biotin onto CoCr-PDA-PEI surface with a density of 0.74 nmol/cm2. The fixation of avidin was demonstrated by water contact angle (WCA) and surface plasmon resonance (SPR). SEM micrograph shows the flexibility of the thin layer coated onto the stent after balloon inflation. Independently of the strategy, a qualitative SEM analysis showed a reduction in platelet activation when the molecule EP224283 was immobilised on avidin. In parallel, the measurement of anticoagulant activity (anti-Xa) revealed a higher anti-factor Xa activity (2.24 IU/mL vs. 0.09 IU/mL in control) when EP224283 was immobilised on avidin. Interestingly, after seven days of degradation, the anticoagulant activity was persistent in both strategies and looked more important with the strategy 2 than in strategy 1.Throughout this work, we developed an innovative vascular stent through the immobilisation of EP224283 onto CoCr-PDA-PEI-(avidin) system, which provides a promising solution to reduce ISR and thrombosis after stent implantation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0928-4931 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8eec816ac60f5ff1bcc7c3763bca4df9 https://hal.univ-lille.fr/hal-02877605 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....8eec816ac60f5ff1bcc7c3763bca4df9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09284931 |
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