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Role of Estradiol in the Regulation of Prolactin Secretion During Late Pregnancy

التفاصيل البيبلوغرافية
العنوان: Role of Estradiol in the Regulation of Prolactin Secretion During Late Pregnancy
المؤلفون: Marta Soaje, Gisela E. Pennacchio, Carlos M Villegas-Gabutti, Graciela A. Jahn
المصدر: Neurochemical Research. 41:3344-3355
بيانات النشر: Springer Science and Business Media LLC, 2016.
سنة النشر: 2016
مصطلحات موضوعية: Receptors, Opioid, mu, Hypothalamus, Middle, PROLACTIN, Biochemistry, 0302 clinical medicine, Pregnancy, MIFEPRISTONE OR RU-486, ESTRADIOL, Progesterone, Estradiol, Naloxone, General Medicine, Mifepristone, Medicina Básica, PREGNANCY, Hormone receptor, Hypothalamus, Female, Receptors, Progesterone, hormones, hormone substitutes, and hormone antagonists, medicine.drug, medicine.medical_specialty, CIENCIAS MÉDICAS Y DE LA SALUD, Tyrosine 3-Monooxygenase, Receptors, Prolactin, medicine.drug_class, Inmunología, 030209 endocrinology & metabolism, 03 medical and health sciences, Cellular and Molecular Neuroscience, Internal medicine, medicine, Animals, Estrogen Receptor beta, NALOXONE, Rats, Wistar, Estrogen receptor beta, business.industry, Receptors, Opioid, kappa, TYROSINE HYDROXYLASE, Estrogen Receptor alpha, Prolactin, Endocrinology, Estrogen, Pregnancy, Animal, RAT, business, Estrogen receptor alpha, 030217 neurology & neurosurgery, Tamoxifen
الوصف: Estrogen action is necessary for evidencing the stimulatory action of mifepristone and naloxone on prolactin (PRL) secretion during late pregnancy. Our aim is to determine the mechanism mediating this facilitator action of estrogens. To investigate the hypothalamic mechanisms involved in estrogen actions in PRL secretion at the end of pregnancy, we measured the effect of pretreatment with the estrogen antagonist tamoxifen on the expression of tyrosine hydroxylase (TH), hormone receptors (ERα and β, PRs, PRLR(long)), and μ- and κ- opioid receptors (ORs) at mRNA (by semiquantitative RT-PCR) and protein (by western blot for TH, PRLR(long), ERα, PRs, μ- and ORs) levels in extracts of medial basal hypothalamus (MBH) and serum PRL, E2 and P4 levels (by RIA) in mifepristone- and naloxone-treated rats. Tamoxifen administration partially prevented PRL release induced by the combined treatment. TH expression diminished and ERα expression increased in mifepristone-treated rats at mRNA and protein levels and tamoxifen partially prevented these changes with no effect on PRs expression. Mifepristone increased PRLR(long) mRNA levels; this increase was blocked by tamoxifen. Combined tamoxifen and mifepristone treatment decreased μ- and k-ORs mRNA but not protein levels. In conclusion, E2 induces neuroadaptive mechanisms necessary to facilitate PRL release preceding delivery. Acting through ERα, E2 modulates hypothalamic dopaminergic neurons activity, regulating TH, μ- and κ-ORs and PRLR(long) expression, and is necessary for evidencing the effects of P4 withdrawal. Its presence on days 14 and 15 of pregnancy is crucial to facilitate the opioid system modulation of PRL secretion at the end of pregnancy in the rat. Fil: Villegas Gabutti, Carlos Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Pennacchio, Gisela Erika. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Jahn, Graciela Alma. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Soaje, Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina
وصف الملف: application/pdf
تدمد: 1573-6903
0364-3190
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8fb38cf9ccba585aad976dcb2dd22ad9
https://doi.org/10.1007/s11064-016-2067-x
حقوق: RESTRICTED
رقم الأكسشن: edsair.doi.dedup.....8fb38cf9ccba585aad976dcb2dd22ad9
قاعدة البيانات: OpenAIRE
الوصف
تدمد:15736903
03643190