Unraveling the features of somatic transposition in the Drosophila intestine
| العنوان: | Unraveling the features of somatic transposition in the Drosophila intestine |
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| المؤلفون: | Annabelle Wurmser, Sonia Lameiras, Allison J. Bardin, Nick Riddiford, Benjamin Boumard, Marius van den Beek, Katarzyna Siudeja, Marine Stefanutti |
| المساهمون: | Génétique et Biologie du Développement, Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Plateforme de sequencage ICGEX, Institut Curie [Paris], HAL-SU, Gestionnaire |
| المصدر: | EMBO Journal EMBO Journal, EMBO Press, 2021, pp.e106388. ⟨10.15252/embj.2020106388⟩ EMBO Journal, 2021, pp.e106388. ⟨10.15252/embj.2020106388⟩ |
| بيانات النشر: | HAL CCSD, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, Transposable element, Small RNA, Somatic cell, [SDV]Life Sciences [q-bio], Retrotransposon, Biology, somatic genome, transposable elements Subject Categories Chromatin, Development, General Biochemistry, Genetics and Molecular Biology, Clonal Evolution, Transposition (music), Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Transcription & Genomics, Intestinal Neoplasms, Animals, Drosophila Proteins, Gene Silencing, Molecular Biology, 030304 developmental biology, Recombination, Genetic, Transcriptionally active chromatin, 0303 health sciences, Receptors, Notch, Whole Genome Sequencing, General Immunology and Microbiology, Sequence Analysis, RNA, Gene Expression Profiling, General Neuroscience, Drosophila midgut, Cell biology, [SDV] Life Sciences [q-bio], Drosophila melanogaster, Organ Specificity, DNA Transposable Elements, Female, Nanopore sequencing, 030217 neurology & neurosurgery |
| الوصف: | International audience; Transposable elements (TEs) play a significant role in evolution, contributing to genetic variation. However, TE mobilization in somatic cells is not well understood. Here, we address the prevalence of transposition in a somatic tissue, exploiting the Drosophila midgut as a model. Using whole-genome sequencing of in vivo clonally expanded gut tissue, we have mapped hundreds of highconfidence somatic TE integration sites genome-wide. We show that somatic retrotransposon insertions are associated with inactivation of the tumor suppressor Notch, likely contributing to neoplasia formation. Moreover, applying Oxford Nanopore longread sequencing technology we provide evidence for tissue-specific differences in retrotransposition. Comparing somatic TE insertional activity with transcriptomic and small RNA sequencing data, we demonstrate that transposon mobility cannot be simply predicted by whole tissue TE expression levels or by small RNA pathway activity. Finally, we reveal that somatic TE insertions in the adult fly intestine are enriched in genic regions and in transcriptionally active chromatin. Together, our findings provide clear evidence of ongoing somatic transposition in Drosophila and delineate previously unknown features underlying somatic TE mobility in vivo. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0261-4189 1460-2075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9192aa62a64a71cb7469a42476dc5c35 https://hal.sorbonne-universite.fr/hal-03154552/file/embj.2020106388.pdf |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....9192aa62a64a71cb7469a42476dc5c35 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 02614189 14602075 |
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