Hypermethylation of IFN-γ in oral cancer tissues
| العنوان: | Hypermethylation of IFN-γ in oral cancer tissues |
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| المؤلفون: | Sheng Xu, Chunyang Jiang, Songbo Tian, Xiaoqin Liu, Huizhen Chen, Dong Ma, Yinghuai Zhang, Yanping Liu, Zhiyong Zhang |
| المصدر: | Clinical Oral Investigations. 21:2535-2542 |
| بيانات النشر: | Springer Science and Business Media LLC, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Pathology, medicine.medical_specialty, Epithelial dysplasia, Carcinogenesis, Enzyme-Linked Immunosorbent Assay, Real-Time Polymerase Chain Reaction, medicine.disease_cause, Interferon-gamma, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, RNA, Messenger, General Dentistry, Neoplasm Staging, business.industry, Cancer, Sequence Analysis, DNA, Methylation, DNA Methylation, Middle Aged, medicine.disease, Primary tumor, Reverse transcriptase, stomatognathic diseases, 030104 developmental biology, 030220 oncology & carcinogenesis, DNA methylation, Carcinoma, Squamous Cell, Cancer research, Female, Mouth Neoplasms, Neoplasm Grading, business, Immunostaining |
| الوصف: | The study aimed to evaluate the methylation pattern of the interferon-gamma (IFN-γ) gene in oral cancer tissues compared with normal and benign oral disease tissues. The oral tissues were gained from the patients of 85 cases of oral squamous cell carcinoma (OSCC), 47 cases of oral dysplastic lesions, and 53 normal biopsies. IFN -γ methylation in oral tissues was verified through methylation-specific polymerase chain reaction (PCR) and DNA sequencing analyses, and the expression levels of IFN-γ messenger RNA (mRNA) and protein were detected using real-time reverse transcription (RT)-PCR and enzyme-linked immunosorbent assays, respectively. IFN-γ was localized in macrophages from oral tissues and detected via immunostaining. IFN-γ mRNA and protein expression levels were evidently decreased in oral cancer tissues, whereas the IFN-γ methylation rate was significantly higher in malignant tumors than in benign and normal tissues (normal, 22.6%; benign, 38.3%; and cancer, 55.3%; P |
| تدمد: | 1436-3771 1432-6981 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::99b2febfff905be36e21627d3b2c4606 https://doi.org/10.1007/s00784-017-2052-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....99b2febfff905be36e21627d3b2c4606 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14363771 14326981 |
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