The Potassium Chloride Cotransporter KCC-2 Coordinates Development of Inhibitory Neurotransmission and Synapse Structure in Caenorhabditis elegans
| العنوان: | The Potassium Chloride Cotransporter KCC-2 Coordinates Development of Inhibitory Neurotransmission and Synapse Structure in Caenorhabditis elegans |
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| المؤلفون: | Michael R. Koelle, Andrew Bellemer, Jessica E. Tanis, James J. Moresco, Biff Forbush |
| المصدر: | The Journal of Neuroscience. 29:9943-9954 |
| بيانات النشر: | Society for Neuroscience, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Sequence Homology, Neurotransmission, Biology, Synaptic Transmission, Chloride, Sodium Potassium Chloride Symporter Inhibitors, Article, gamma-Aminobutyric acid, Receptors, G-Protein-Coupled, Synapse, Sexual Behavior, Animal, Chlorides, Furosemide, medicine, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Motor Neurons, Symporters, Muscles, General Neuroscience, Up-Regulation, Cell biology, Hypotonic Solutions, Biochemistry, Mutation, Synapses, Chloride channel, Synaptic Vesicles, Cotransporter, Synapse maturation, medicine.drug |
| الوصف: | Chloride influx through GABA-gated chloride channels, the primary mechanism by which neural activity is inhibited in the adult mammalian brain, depends on chloride gradients established by the potassium chloride cotransporter KCC2. We used a genetic screen to identify genes important for inhibition of the hermaphrodite-specific motor neurons (HSNs) that stimulateCaenorhabditis elegansegg-laying behavior and discovered mutations in a potassium chloride cotransporter,kcc-2. Functional analysis indicates that, like mammalian KCCs,C. elegansKCC-2 transports chloride, is activated by hypotonic conditions, and is inhibited by the loop diuretic furosemide. KCC-2 appears to establish chloride gradients required for the inhibitory effects of GABA-gated and serotonin-gated chloride channels onC. elegansbehavior. In the absence of KCC-2, chloride gradients appear to be altered in neurons and muscles such that normally inhibitory signals become excitatory.kcc-2is transcriptionally upregulated in the HSN neurons during synapse development. Loss of KCC-2 produces a decrease in the synaptic vesicle population within mature HSN synapses, which apparently compensates for a lack of HSN inhibition, resulting in normal egg-laying behavior. Thus, KCC-2 coordinates the development of inhibitory neurotransmission with synapse maturation to produce mature neural circuits with appropriate activity levels. |
| تدمد: | 1529-2401 0270-6474 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0bba1b508023c728fcbdde78a02fb98 https://doi.org/10.1523/jneurosci.1989-09.2009 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a0bba1b508023c728fcbdde78a02fb98 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15292401 02706474 |
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