Studies on the effect of Celastrus orbiculatus (Celastraceae) extract on chemosensitivity of liver cancer cells via Wnt/β-catenin pathway
| العنوان: | Studies on the effect of Celastrus orbiculatus (Celastraceae) extract on chemosensitivity of liver cancer cells via Wnt/β-catenin pathway |
|---|---|
| المؤلفون: | Xiaobo Ding, Chengming Jiao, Yunfei Lu, Qiting Huang, Laijun Song |
| المصدر: | Tropical Journal of Pharmaceutical Research; Vol. 20 No. 10 (2021); 2077-2082 |
| بيانات النشر: | African Journals Online (AJOL), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, Chemistry, Cell growth, Cell, Wnt signaling pathway, Pharmaceutical Science, Pharmacology, biology.organism_classification, Hep G2, Celastrus orbiculatus, Cyclin D1, medicine.anatomical_structure, Cell culture, Apoptosis, medicine, Pharmacology (medical), Celastrus orbiculatus, Liver cancer, Chemosensitivity, Chemotherapy, Apoptosis, Cell invasion |
| الوصف: | Purpose: To examine the efficacy of Celastrus orbiculatus extract (COE) on the chemosensitivity of liver cancer (LC) cells and its mechanism of action.Methods: Hep G2/ADM cells in the logarithmic growth phase were assigned to a control group (no treatment for cell culture medium only) and a study group (120 μg/ml COE added to the culture medium). After 48 h of incubation, the biological responses were compared. The study group wasdivided into groups A and B, while control group was divided into groups C and D, with 1 μmol/L XAV939 added in groups A and C. Cell proliferation, cell invasion, cell apoptosis rate, and apoptosis protein in the four groups were evaluated.Results: The study group showed significantly lower values in terms of cell proliferation and cell invasiveness (p < 0.05) and a higher apoptotic rate than the control group (p < 0.05)). The study group also demonstrated an elevated pro-apoptotic protein Bax level and a declined anti-apoptotic protein Bcl-2 level. In contrast to group B, the proliferation and invasiveness of Hep G2/ADM cells in group A treated with the inhibitor, XAV939, were significantly lower (p < 0.05), while the apoptotic rate exhibited a significant increase (p < 0.05). There was a rise in the level of pro-apoptotic protein, Bax, and a fall in the anti-apoptotic protein Bcl-2 level in group A. Lower levels of β-catenin, c-Myc, and cyclin D1 protein were observed in the study group compared with the control group (p < 0.05). Compared with other groups, the multiplication capacity and invasiveness of cells in group A treated with COE and inhibitor XAV939 significantly declined, while the apoptotic rate increased (p < 0.05).Conclusion: COE reverses drug resistance in chemotherapy by inhibiting the expression of Wnt/β-catenin pathway in LC cells. Therefore, COE has potentials for use along with chemotherapeutic agents in the management of liver cancer. |
| وصف الملف: | application/pdf |
| تدمد: | 1596-9827 1596-5996 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0c4862f3292756f509d3b47746ec74d https://doi.org/10.4314/tjpr.v20i10.10 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a0c4862f3292756f509d3b47746ec74d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15969827 15965996 |
|---|