Generation of multipotent cell lines from a distinct population of male germ line stem cells
| العنوان: | Generation of multipotent cell lines from a distinct population of male germ line stem cells |
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| المؤلفون: | Francisco J. Silva, Thomas Ramos, Rafael Gonzalez, Tracy Wang, Kyle Howerton, Chauncey B. Sayre, Francis Pau, Natalia Slepko, Joel Marh, Fariborz Izadyar |
| المصدر: | REPRODUCTION. 135:771-784 |
| بيانات النشر: | Bioscientifica, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, Homeobox protein NANOG, Embryology, Cellular differentiation, Green Fluorescent Proteins, Mice, Nude, Mice, Transgenic, Embryoid body, Biology, Mice, Endocrinology, SOX2, medicine, Animals, Humans, Cell Lineage, Promoter Regions, Genetic, Telomerase, Cells, Cultured, Chimera, Multipotent Stem Cells, Stem Cells, Teratoma, Obstetrics and Gynecology, Cell Biology, Embryonic stem cell, Molecular biology, Spermatogonia, Cell biology, Proto-Oncogene Proteins c-kit, medicine.anatomical_structure, Reproductive Medicine, Karyotyping, Stem cell, Genetic Engineering, Octamer Transcription Factor-3, Biomarkers, Germ cell, Stem Cell Transplantation, Adult stem cell |
| الوصف: | Spermatogonial stem cells (SSCs) maintain spermatogenesis by self-renewal and generation of spermatogonia committed to differentiation. Under certainin vitroconditions, SSCs from both neonatal and adult mouse testis can reportedly generate multipotent germ cell (mGC) lines that have characteristics and differentiation potential similar to embryonic stem (ES) cells. However, mGCs generated in different laboratories showed different germ cell characteristics, i.e., some retain their SSC properties and some have lost them completely. This raises an important question: whether mGC lines have been generated from different subpopulations in the mouse testes. To unambiguously identify and track germ line stem cells, we utilized a transgenic mouse model expressing green fluorescence protein under the control of a germ cell-specificPou5f1(Oct4) promoter. We found two distinct populations among the germ line stem cells with regard to their expression of transcription factorPou5f1and c-Kit receptor. Only the POU5F1+/c-Kit+ subset of mouse germ line stem cells, when isolated from either neonatal or adult testes and cultured in a complex mixture of growth factors, generates cell lines that express pluripotent ES markers, i.e.,Pou5f1,Nanog,Sox2,Rex1,Dppa5,SSEA-1, and alkaline phosphatase, exhibit high telomerase activity, and differentiate into multiple lineages, including beating cardiomyocytes, neural cells, and chondrocytes. These data clearly show the existence of two distinct populations within germ line stem cells: one destined to become SSC and the other with the ability to generate multipotent cell lines with some pluripotent characteristics. These findings raise interesting questions about the relativity of pluripotency and the plasticity of germ line stem cells. |
| تدمد: | 1741-7899 1470-1626 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3e663f0c2262003b67788a27d60bcd1 https://doi.org/10.1530/rep-07-0479 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a3e663f0c2262003b67788a27d60bcd1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17417899 14701626 |
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