p63 regulates glutaminase 2 expression
| العنوان: | p63 regulates glutaminase 2 expression |
|---|---|
| المؤلفون: | Massimilano Agostini, Alessandro Finazzi Agrò, Lucilla Bongiorno-Borbone, Alessandro Terrinoni, Arnold J. Levine, Lello Zolla, Daniel A. Notterman, Angelo Peschiaroli, Arianna Giacobbe, Gerry Melino, Zhaohui Feng, Francesca Bernassola, Elke Markert |
| المصدر: | Cell cycle (Georget. Tex.) 12 (2013): 1395–1405. doi:10.4161/cc.24478 info:cnr-pdr/source/autori:Giacobbe Arianna1; Bongiorno-Borbone Lucilla1; Bernassola Francesca1; Terrinoni Alessandro2; Markert Elke Katrin3; Levine Arnold J.3; Feng Zhaohui4; Agostini Massimiliano5; Zolla Lello6; Finazzi Agrò Alessandro1; Notterman Daniel A.7, Melino Gerry8,9; Peschiaroli Angelo10/titolo:p63 regulates glutaminase 2 expression/doi:10.4161%2Fcc.24478/rivista:Cell cycle (Georget. Tex.)/anno:2013/pagina_da:1395/pagina_a:1405/intervallo_pagine:1395–1405/volume:12 |
| بيانات النشر: | Landes Bioscience, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Gene isoform, Keratinocytes, glutaminolysis, Physiological, Cellular differentiation, Apoptosis, colon carcinoma, Biology, Adenocarcinoma, Stress, medicine.disease_cause, Cell Line, Downregulation and upregulation, Glutaminase, Stress, Physiological, Report, Cell Line, Tumor, medicine, Humans, Settore BIO/10, Skin, DNA Damage, Cytoprotection, Cell Differentiation, skin differentiation, Reactive Oxygen Species, Tumor Suppressor Proteins, p63, Transcription Factors, Up-Regulation, Colonic Neoplasms, Histone Deacetylase Inhibitors, Molecular Biology, Transcription factor, Tumor, Settore BIO/11, Promoter, Cell Biology, Molecular biology, Cancer cell, Carcinogenesis, Developmental Biology |
| الوصف: | The transcription factor p63 is critical for many biological processes, including development and maintenance of epidermal tissues and tumorigenesis. Here, we report that the TAp63 isoforms regulate cell metabolism through the induction of the mitochondrial glutaminase 2 (GLS2) gene both in primary cells and tumor cell lines. By ChIP analysis and luciferase assay, we confirmed that TAp63 binds directly to the p53/p63 consensus DNA binding sequence within the GLS2 promoter region. Given the critical role of p63 in epidermal differentiation, we have investigated the regulation of GLS2 expression during this process. GLS2 and TAp63 expression increases during the in vitro differentiation of primary human keratinocytes, and depletion of GLS2 inhibits skin differentiation both at molecular and cellular levels. We found that GLS2 and TAp63 expression are concomitantly induced in cancer cells exposed to oxidative stresses. siRNA-mediated depletion of GLS2 sensitizes cells to ROS-induced apoptosis, suggesting that the TAp63/GLS2 axis can be functionally important as a cellular antioxidant pathway in the absence of p53. Accordingly, we found that GLS2 is upregulated in colon adenocarcinoma. Altogether, our findings demonstrate that GLS2 is a bona fide TAp63 target gene, and that the TAp63-dependent regulation of GLS2 is important for both physiological and pathological processes. |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4f5bf0d19677a8a6ee6f37baeb9878f https://europepmc.org/articles/PMC3674067/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a4f5bf0d19677a8a6ee6f37baeb9878f |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |