Identification of Myocardial and Vascular Precursor Cells in Human and Mouse Epicardium
| العنوان: | Identification of Myocardial and Vascular Precursor Cells in Human and Mouse Epicardium |
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| المؤلفون: | Luca Battistini, Giulio Pompilio, Maurizio C. Capogrossi, Antonella Zacheo, Vincenzo Loiaconi, Federica Limana, Roberta De Mori, Giovanna Borsellino, Massimo Santini, Antonella Mangoni, David Mocini, Elisa Vigna, Adamo Diamantini, Antonia Germani |
| المصدر: | Circulation Research. 101:1255-1265 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Physiology, Population, Cell, CD34, Biology, Stem cell marker, Mice, Fetal Heart, Cell Movement, Precursor cell, medicine, Animals, Humans, Myocytes, Cardiac, education, education.field_of_study, Myocardium, Stem Cells, Mesenchymal stem cell, Mice, Inbred C57BL, Endothelial stem cell, medicine.anatomical_structure, cardiovascular system, Female, Endothelium, Vascular, Stem cell, Cardiology and Cardiovascular Medicine, Pericardium |
| الوصف: | During cardiac development, the epicardium is the source of multipotent mesenchymal cells, which give rise to endothelial and smooth muscle cells in coronary vessels and also, possibly, to cardiomyocytes. The aim of the present study was to determine whether stem cells are retained in the adult human and murine epicardium and to investigate the regenerative potential of these cells following acute myocardial infarction. We show that c-kit + and CD34 + cells can indeed be detected in human fetal and adult epicardium and that they represent 2 distinct populations. Both subsets of cells were negative for CD45, a cell surface marker that identifies the hematopoietic cell lineage. Immunofluorescence revealed that freshly isolated c-kit + and CD34 + cells expressed early and late cardiac transcription factors and could acquire an endothelial phenotype in vitro. In the murine model of myocardial infarction, there was an increase in the absolute number and proliferation of epicardial c-kit + cells 3 days after coronary ligation; at this time point, epicardial c-kit + cells were identified in the subepicardial space and expressed GATA4. Furthermore, 1 week after myocardial infarction, cells coexpressing c-kit + , together with endothelial or smooth muscle cell markers, were identified in the wall of subepicardial blood vessels. In summary, the postnatal epicardium contains a cell population with stem cell characteristics that retains the ability to give rise to myocardial precursors and vascular cells. These cells may play a role in the regenerative response to cardiac damage. |
| تدمد: | 1524-4571 0009-7330 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a50acbbd169f61efb9bf22c7f1cc253f https://doi.org/10.1161/circresaha.107.150755 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....a50acbbd169f61efb9bf22c7f1cc253f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15244571 00097330 |
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