Localization of the Domains of the Haemophilus ducreyi Trimeric Autotransporter DsrA Involved in Serum Resistance and Binding to the Extracellular Matrix Proteins Fibronectin and Vitronectin
| العنوان: | Localization of the Domains of the Haemophilus ducreyi Trimeric Autotransporter DsrA Involved in Serum Resistance and Binding to the Extracellular Matrix Proteins Fibronectin and Vitronectin |
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| المؤلفون: | Isabelle Leduc, Bonnie Olsen, Christopher Elkins |
| المصدر: | Infection and Immunity. 77:657-666 |
| بيانات النشر: | American Society for Microbiology, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Molecular Sequence Data, Immunology, Plasma protein binding, Biology, Microbiology, Bacterial Adhesion, Haemophilus ducreyi, Bacterial Proteins, Amino Acid Sequence, Vitronectin, Gene Expression Profiling, Binding protein, Cell Membrane, Gene Expression Regulation, Bacterial, biology.organism_classification, Molecular Pathogenesis, Extracellular Matrix, Fibronectins, Protein Structure, Tertiary, Fibronectin, Bacterial adhesin, Infectious Diseases, Fibronectin binding, biology.protein, Parasitology, Extracellular matrix protein binding, Carrier Proteins, Gene Deletion, Protein Binding |
| الوصف: | Resisting the bactericidal activity of naturally occurring antibodies and complement of normal human serum is an important element in the evasion of innate immunity by bacteria. In the gram-negative mucosal pathogen Haemophilus ducreyi , serum resistance is mediated primarily by the trimeric autotransporter DsrA. DsrA also functions as an adhesin for the extracellular matrix proteins fibronectin and vitronectin and mediates attachment of H. ducreyi to keratinocytes. We sought to determine the domain(s) of the 236-residue DsrA protein required for serum resistance and extracellular matrix protein binding. A 140-amino-acid truncated protein containing only the C-terminal portion of the passenger domain and the entire translocator domain of DsrA exhibited binding to fibronectin and vitronectin and conferred serum resistance to an H. ducreyi serum-sensitive strain. A shorter DsrA construct consisting of only 128 amino acids was unable to bind to extracellular matrix proteins but was serum resistant. We concluded that neither fibronectin binding nor vitronectin binding is required for high-level serum resistance in H. ducreyi . |
| تدمد: | 1098-5522 0019-9567 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aada463852364f96d6d8a7b646180129 https://doi.org/10.1128/iai.00819-08 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....aada463852364f96d6d8a7b646180129 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985522 00199567 |
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