Time-course changes in 5-fluorouracil-induced neural progenitor cell damages in the developing rat brain

التفاصيل البيبلوغرافية
العنوان: Time-course changes in 5-fluorouracil-induced neural progenitor cell damages in the developing rat brain
المؤلفون: Yachiyo Fukunaga, Toru Hoshiya, Yuko Yamaguchi, Kazutoshi Tamura, Tsubasa Saito, Mizuho Takagi
المصدر: Journal of Toxicologic Pathology
بيانات النشر: Japanese Society of Toxicologic Pathology, 2021.
سنة النشر: 2021
مصطلحات موضوعية: Fetus, 5-Fluorouracil, Cell cycle, Biology, Toxicology, Neural stem cell, Pathology and Forensic Medicine, Andrology, neural progenitor cell damage, Apoptosis, embryonic structures, parasitic diseases, immunohistochemistry, time-course change, histopathology, Immunohistochemistry, Original Article, Progenitor cell, Mitosis, Pyknosis
الوصف: 5-Fluorouracil (5-Fu) is a DNA-damaging agent and teratogenic in rodents. This study aimed to investigate its influence on neural progenitor cells (NPCs) in the developing fetal rat brain. Dams were intraperitoneally injected with 5-Fu (50 mg/kg b.w.) on gestation day 13 and its effects on fetal NPCs were observed from 3 to 72 hours after treatment (HAT), via periodic examination at six intervals. In NPCs of the fetal brain, the p53-labeling index (LI%) was markedly elevated at 3 HAT. Pyknosis and cleaved caspase-3-LI% also increased at 3 HAT, reaching peak values at 9 and 12 HAT. These parallel changes suggested the induction of apoptosis through a p53-mediated pathway. Pyknotic NPCs were distributed across the ventricular zone (VZ) of the telencephalic wall until 12 HAT, and became localized in the medial and dorsal layers at 12 and 48 HAT. Significant decreases in the numbers of mitotic NPCs and BrdU-LI% were noted from 3 HAT and 24 HAT, respectively. BrdU-positive NPCs were located in the ventral and middle layer at 24 and 48 HAT. p21-positive cells were detected at 12 and 24 HAT. The present results demonstrated that p53-mediated apoptosis was induced in all phases of the cell cycle of the NPCs in the early stage after 5-FU treatment. Furthermore, apoptosis of NPCs and suppression of cell proliferative activity are the events that take place in parallel leading to prominent reduction in the width of the telencephalic wall.
اللغة: English
تدمد: 1881-915X
0914-9198
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab10b353094774b97bcc826e92742a98
http://europepmc.org/articles/PMC8484929
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....ab10b353094774b97bcc826e92742a98
قاعدة البيانات: OpenAIRE